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油田产出液中产甲烷菌的分离与特性初步研究 被引量:5

Separation and Characterization of Methanogenus in Produced Fluids of Oilfield
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摘要 采用改良的Hungate厌氧技术以新疆油田区块采出水为接种物,分离出一株产甲烷菌,并评价利用其生理活动及代谢产物来提高原油采收率。该菌经过分离后进行液体纯培养,500ml培养液培养4周后累计产气量达300ml,气相成分经气相色谱检测为甲烷。这在油藏中能够增加地层压力,有利于提高微生物采油效果。 Methanogenic bacteria strain was extremely anaerobe,which played an important role in global carbon cycle,and it attracted more and more researchers interests.In this paper,a methanogen was separated and identifited by the application of improved Hungate roll-tube technique in produced fluids of Xinjiang oilfield.The production of CH4 is steady in liquid medium.As microbial enhanced oil recovery is concerned,the reservoir of methane-producing archaea can increase metabolic activity of formation pressure,dissolved in the crude oil to reduce viscosity,enhance oil recovery.
出处 《长江大学学报(自科版)(上旬)》 CAS 2009年第01X期164-166,共3页 JOURNAL OF YANGTZE UNIVERSITY (NATURAL SCIENCE EDITION) SCI & ENG
关键词 产甲烷菌 采出液 厌氧 提高石油采收率 methanogenic bacteria anaerobic cultivation oilfield produced fluids microbial enhanced oil recovery
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  • 1黎霞,承磊,邓宇,张辉.石油烃厌氧生物降解研究进展[J].应用与环境生物学报,2008,14(2):283-289. 被引量:13
  • 2张万芹,康冀川,王开功,黄庆华.产甲烷细菌的筛选及培养技术优化研究[J].西南农业学报,2007,20(6):1207-1210. 被引量:1
  • 3承磊,仇天雷,邓宇,张辉.油藏厌氧微生物研究进展[J].应用与环境生物学报,2006,12(5):740-744. 被引量:25
  • 4公维佳,李文哲,刘建禹.厌氧消化中的产甲烷菌研究进展[J].东北农业大学学报,2006,37(6):838-841. 被引量:45
  • 5刘亭亭,曹靖瑜.产甲烷菌的分离及其生长条件研究[J].黑龙江水专学报,2007,34(4):120-122. 被引量:22
  • 6Shestakova N M, Grigoryan A A, Qingxian Feng, et al. Characterization of the methanogenic community from the high-temperature Dagang oil field (P. R. China) . In: New Research on the Environment and Biotechnology. Ed. : A.E. Kuznetsov and G. E. Zaikov, Nova Science Publishers, Inc. , New York. 2006. 159-174.
  • 7Nazina T, Shestakova N, Grigor yan A, et a l. Phylogenetic diversity and activity of anaerobic microorganisms of high-temperature horizons of the Dagang oil field [J] . Microbiology (Moscow), 2006, 75: 55-65.
  • 8Grigoryan A A, Voordouw G. Microbiology to help solve our energy needs: Methanogenesis from oil and impact of nitrate on the oil field sulfur cycle [A]. Wiegel J, Maier R, Adams M. Incredible Anaerobes: From Physiology to Genomics to Fuels [C] . New York: New York Academy of Sciences, 2008.
  • 9Balch W E, Wolfe R S. New Approach to the Cultivation of Methanogenic Bacteria: 2-mercaptoethanesulfonic Acid (HS-CoM) dependent Growth of Methanobacterium Rumi-nantium in a Pressureized Atmosphere [J]. Applied and Environmental Microbiology, 1976, 32 (6) : 781-791.

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