摘要
采用改良的Hungate厌氧技术以新疆油田区块采出水为接种物,分离出一株产甲烷菌,并评价利用其生理活动及代谢产物来提高原油采收率。该菌经过分离后进行液体纯培养,500ml培养液培养4周后累计产气量达300ml,气相成分经气相色谱检测为甲烷。这在油藏中能够增加地层压力,有利于提高微生物采油效果。
Methanogenic bacteria strain was extremely anaerobe,which played an important role in global carbon cycle,and it attracted more and more researchers interests.In this paper,a methanogen was separated and identifited by the application of improved Hungate roll-tube technique in produced fluids of Xinjiang oilfield.The production of CH4 is steady in liquid medium.As microbial enhanced oil recovery is concerned,the reservoir of methane-producing archaea can increase metabolic activity of formation pressure,dissolved in the crude oil to reduce viscosity,enhance oil recovery.
出处
《长江大学学报(自科版)(上旬)》
CAS
2009年第01X期164-166,共3页
JOURNAL OF YANGTZE UNIVERSITY (NATURAL SCIENCE EDITION) SCI & ENG
关键词
产甲烷菌
采出液
厌氧
提高石油采收率
methanogenic bacteria
anaerobic cultivation
oilfield produced fluids
microbial enhanced oil recovery