摘要
用响应面法对微球菌DS16发酵生产微生物絮凝剂的条件进行了优化。首先通过Plackett-Burman设计从影响絮凝活性的8个因素中筛选出具有显著活性效应的因素:酵母提取物、液糖、(NH4)2SO4、MnCl2。然后用响应面设计对这四个因素进行优化分析,获得了这几个因素的最佳浓度,确定了发酵培养基的最优配方(g/L):液糖20、K2HPO43、NaH2PO41.8、(NH4)2SO44.3、酵母提取物0.9、MgSO40.2、CaCl20.1、MnCl20.004。利用优化的培养基进行发酵,絮凝活性提高了16.44%。
Fermentation condition optimization of Micrococcus DS16 for microbial flocculant production was studied. Firstly, the four distinguished factors, yeast extract, liquid sugar, （NH4）2SO4, MnCl2,were screened from 8 affecting ones to flocculating activity by Plackett-Burman design. Then the RSM was used to optimize above four factors and as a result,the optimal concentrations of the variables were obtained, and the optimal component proportions of medium were further confirmed, that is （g/L） ： liquid sugar 20, K2 HPO4 3, Nail2 PO4 1.8, （NH4） z SO4 4.3, yeast extract 0. 9, Mg2 SO4 0. 2, CaCl2 0.1,MnCl2 0. 004. Using the optimized medium to ferment microbial flocculant,its flocculating activity was increased by 16.44%.
出处
《化工科技》
CAS
2009年第4期17-22,共6页
Science & Technology in Chemical Industry
