摘要
为了检测神经毒剂类似物甲基膦酸二甲酯(DMMP),制备了一种新型的固定化植物酯酶-单磺酸基四苯基卟啉(TPPS1)纳米传感膜.首先对膜材制备的影响因素,如戊二醛浓度、活化时间和吸附时间等进行了优化研究.再通过原子力显微镜(AFM)和UV-Vis光谱对在优化条件下制备的膜材的表面形貌和对DMMP的传感性能分别进行了表征和分析.结果表明,当戊二醛浓度为0.43mol/L,活化时间为80min,吸附时间为170min时,固定化植物酯酶的活力较高.该膜材的纳米结构增加了其对DMMP的敏感性,降低了检测限.TPPS1与固定化植物酯酶作用后会在422nm处形成一特征峰.把固定化植物酯酶-TPPS1表面暴露于DMMP中,由于DMMP可以把卟啉从酶的活性位点上置换下来,所以引起422nm处吸光度的降低.并且,在DMMP浓度低于9×10-7mol/L的范围内,422nm处吸光度的变化与DMMP的浓度呈线性相关.该纳米传感膜可以检测浓度低至4.5×10-10mol/L的DMMP.
A novel sensing nano-film immobilized plant-esterase-monosulfonate tetraphenyl porphyrin （ TPPS1 ） was prepared for the detection of dimethyl methyl phosphonate （ DMMP）, a stimulant of nerve agents. Several factors that influence preparation of the film, such as sodium alginate concentration, activation time, and adsorption time, were investigated. The morphology and sensing property to DMMP of the film prepared under optimized conditions were characterized and analyzed by atomic force microscope （AFM） and UV-Vis spectroscopy, respectively. The results show that the activity of immobilized plantesterase is higher when sodium alginate concentration is 0. 43 mol/L, activation time is 80 min, and adsorption time is 170 min. The nano-structure of the film had a good effect on its sensitivity to DMMP and lowered the detection limit. The interaction of TPPS1 with immobilized plant-esterase yielded a characteristic peak at 422 nm. Exposure of the immobilized plant-esterase-TPPS1 surface to DMMP resulted in a decrease in absorbance intensity at 422 nm due to the displacement of the porphyrin from the active site by DMMP. The loss in absorbance intensity at 422 nm was linearly dependent on DMMP concentration below 9 × 10 ^-7 mol/L. DMMP concentration as low as 4.5 × 10 ^-10 mol/L can be detected by the sensing nano-film.
出处
《纳米技术与精密工程》
EI
CAS
CSCD
2009年第4期300-304,共5页
Nanotechnology and Precision Engineering
基金
国家自然科学基金资助项目(30770568)
国家高技术研究发展计划(863)项目(2006AA04Z349)
重庆市自然科学基金资助项目(2008AB2024
2008AC7037)
重庆大学研究生科技创新基金资助项目(200707A1B0180255)
