摘要
目的探讨甲状腺乳头状癌(PTC)中血管内皮生长因子-C(VEGF-C)的表达、肿瘤相关巨噬细胞(TAMs)浸润与淋巴管生成的关系及临床意义。方法采用免疫组化SP法检测72例PTC中VEGF-C、血管内皮生长因子-C受体3(VEGFR-3,标记淋巴管)、CD68(标记TAMs)的表达;在显微镜下计数淋巴管密度(LVD)、TAMs;采用免疫组化双染LAB-SA法观察TAMs中VEGF-C的表达。结果VEGF-C阳性指数(PI)值腺外型(21.4±2.8)高于微癌(10.1±0.8)与腺内型(11.9±1.2)(P均<0.05),有淋巴结转移(23.2±3.8)显著高于无淋巴转移(14.5±2.9)(P<0.01);TAMs计数腺外型(43.2±5.6)高于微癌(18.8±1.9)与腺内型(23.0±2.0)(P均<0.01),有淋巴结转移(41.3±8.4)显著高于无淋巴转移(30.4±4.8)(P<0.01);LVD腺内型(9.9±1.8)高于微癌(6.0±0.8)、腺外型(19.2±3.0)高于腺内型(P均<0.05),有淋巴结转移(19.6±2.5)高于无淋巴转移(12.5±2.8)(P<0.05)。VEGF-C与LVD计数呈正相关(r=0.91,P<0.01),TAMs与LVD呈正相关(r=0.83,P<0.01)。VEGF-C阳性表达的巨噬细胞占总巨噬细胞的21.3%。结论VEGF-C、TAMs及淋巴管生成与PTC的侵袭及淋巴结转移有关;VEGF-C、TAMs参与了淋巴管生成。
Objective To investigate the relationships in expression of VEGF-C,tumor associated macrophages (TAMs) infiltration, ]ymphangiogenesis and clinical significance in papillary thyroid eareinoma (PTC). Methods Expression of VEGF-C, CD68 ( marking TAMs) and VEGFR-3 ( marking lymphatic) was determined by immunohistoehemical SP method in 72 cases with PTC. Double staining immunohistochemistry ( LAB SA method) was applied to observe the expression of VEGFc in TAMs. Results The VEGF-C PI in EPC type ( 21.4 ± 2.8 ) was obviously higher than that of PMC type ( 10.1 ± 0.8) and IPC type ( 11.9 ±1.2) ( P 〈 0.05 ), and it was obviously higher in patients with lymph node metastasis (23.2 ± 3.8 ) than that of without metastasis ( 14.5 ± 2.9, P 〈 0.01 ). The TAMs count in EPC type (43.2 ± 5.6) was obviously higher than that of PMC type (18.8 ± 1.9) and IPC type (23.0 ± 2.0) ( P 〈 0.01 ), and it was higher in patients with lymph node metastasis(41.3 ± 8.4)than that of without metastasis ( 30.4± 4.8 ) ( P 〈 0.01 ). Additionally, the LVD was (6.0 ± 0.8 ), (9.9 ± 1.8 ) and ( 19.2 ± 3.0) in PMC, iPC and EPC type, respectively, and it had significant differences among groups ( P 〈 0.05). The LVD in patients with lymph node metastasis( 19.6 ± 2.5) was higher than that of without metastasis( 12.5 ±2.8) ( P 〈 0.05). There was positive correlation between VEGF-C and LVD( r = 0.91, P 〈 0.01 ), TAMs and LVD ( r = 0.83, P 〈 0.01 ). Macrophage with positive VEGF-C expression aceounted for 21.3%. Conclusion It demonstrated that VEGF-C, TAMs and lymphangiogenesis were related to invasion and metastasis of PTC, furthermore, it showed that VEGF-C and TAMs played important roles in lymphangiogenesis.
出处
《疑难病杂志》
CAS
2009年第10期599-601,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
关键词
甲状腺乳头状癌
血管内皮生长因子-C
肿瘤相关巨噬细胞
Papillary thyroid eareinoma
Vascular endothelial growth faetor-C
Tumor assoeiated maerop hages