摘要
目的:探讨从涿鹿柳蒿中提取多糖的方法并测定多糖含量。方法:采用水提醇沉法提取柳蒿粗多糖,无水乙醇、丙酮、石油醚依次洗涤纯化得柳蒿多糖;以苯酚-硫酸显色法显色,分光光度法在波长490nm处测定其含量。结果:柳蒿中多糖含量为5.8mg.g-1生药,加样回收率98.9%,RSD2.2%。结论:本方法提取方便,测定简便,灵敏度高,重复性好。
Objective:To distill Polysaccharides in Artemisia selengensis Turcz. and determine polysaccharides content. Methods: Adopt water solution and alcohol sedimentation were sublimated by absolute alcohol,acetone and petroleum ether, content was determined through the photometric and phenol - sulfuric acid method with detection wavelength at 490nm. Results: Artemisia selengensis Turcz. polysaccharides content 5.8mg · g^-1 crude drugs,the recovery of assay was 99.4% ,RSD=2.2%. Conclusion:The method developed is simple,easy for determination with high sensitivity,good reproducibility.
出处
《河北北方学院学报(医学版)》
2009年第5期37-39,共3页
Journal of Hebei North University:Medical Edition
关键词
柳蒿
多糖类
苯酚-硫酸法
Artemisia Selengensis Turcz. , Polysacharides,Phenol-sulfurie acid method