摘要
目的探讨肝衰竭患者外周血单核细胞（PBMC）Toll样受体2（TLR2）和TLR4表达的变化及其在肝衰竭发病机制中的作用。方法采用RT—PCR法检测健康对照组（20名）、慢性乙型肝炎组（CHB，20例）、肝衰竭早期组（18例）和肝衰竭中晚期组（14例）患者PBMC中TLR2 mRNA和TLR4 mRNA的表达。ELISA法检测患者血中内毒素、TNF-α和IL-6的水平。结果与健康对照组比较，CHB组、肝衰竭早期组和中晚期组患者PBMC中的TLR2 mRNA、TLR4 mRNA，以及血中内毒素、TNF-α和IL-6水平有所上升，差异具有统计学意义（F值分别为32．997、37．476、23．951、57．265和38．403，P值均〈0．01）。TLR2 mRNA的表达在肝衰竭中晚期组中高于早期组，而TLR4 mRNA的表达则相反。在肝衰竭早期和中晚期，TLR2 mRNA的表达与TNF-α、IL-6均有明显相关性（FTNF为0．865和0．921，rIL-6为0．762和0．851，P值均〈0．01），TLR4 mRNA在肝衰竭早期与TNF—α和IL-6具有相关性（r值分别为0．917和0．788，P值均〈0．01）。结论TLR2和TLR4介导的炎性反应可能参与了肝衰竭的发病机制。
Objective To investigate the expression of Toll-like receptor 2 and 4 （TLR2 and TLR4） in peripheral blood mononuelear cells （PBMCs） and the serum inflammatory cytokine levels in patients with liver failure. Methods The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were detected by RT-PCR in 20 healthy controls, 20 chronic hepatitis B （CHB） patients, 18 liver failure patients in early stage and 14 in intermediate-end stage. The serum contents of endotoxin, TNF-α and IL-6 were detected by ELISA. Results Compared with the healthy controls, the expression of TLR2 mRNA and TLR4 mRNA, and the contents of endotoxin, TNF-α and IL-6 increased in CHB patients and liver failure patients （both early stage and intermediate-end stage） （F = 32. 997, 37. 476, 23. 951, 57. 265 and 38. 403, P 〈 0. 01 ）. TLR2 mRNA expression in liver failure patients in intermediate-end stage was higher than that in the early stage, but that for TLR4 mRNA was lower than that in early stage. The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were significantly correlated with the contents of TNF-α and IL-6 （ r = 0. 917 and 0. 788, P 〈 0.01 ）. Conclusion The inflammation reaction mediated by TLR2 and TLR4 might participate in the pathogenesis of liver failure.
出处
《中华临床感染病杂志》
CAS
2009年第4期197-200,共4页
Chinese Journal of Clinical Infectious Diseases
