摘要
将筛选得到的黑曲霉(Aspergillus niger)ZJB-09101菌株应用于外消旋环氧氯丙烷的手性拆分,对菌种产环氧化物水解酶的发酵条件和外消旋环氧氯丙烷的手性催化过程进行了研究。结果表明,黑曲霉ZJB-09101产环氧化物水解酶的最佳培养基成分是:淀粉14.63g/L,豆饼粉6.45g/L,K2HPO·43H2O0.8g/L,KH2PO4·3H2O0.4g/L,MgSO4·7H2O0.05g/L,pH7.0。ZJB-09101菌株在150r/min、30℃条件下,经过4d的培养,环氧化物水解酶的酶活达90.36U/L,比优化前的39.27U/L提高了130%。在环己烷的反应体系中,底物外消旋环氧氯丙烷浓度为0.6%,在30℃下生物催化反应7h,(S)-环氧氯丙烷的e.e.值和产率分别为99.06%和17.14%。
The isolated strain, Aspergillus niger ZJB-090101, was employed in this work for enzyme resolution of racemie epichlorohydrin. The cultivation conditions of A. niger ZJB-09101 for production of epoxide hydrolase, and the enzymatic characteristics of epoxide hydrolase were investigated. The results showed that the optimal medium for enzyme production was as follows： starch 14.63 g/L, soybean cake 6.45 g/L, KH2PO4 0.4 g/L, K2HPO4 0.8 g/L, MgSO4 .7H20 0.05 g/L, and initial culture pH was 7.0. The maximal enzyme activity of 90.36 U/L was obtained after 4 days cultivation at 30℃ with 150 r/min, and increased by 130% as compared with that of unoptimized culture condition. The enzymatic resolution was carried out at cyclohexane solution with 0.6% of racemic epichlorohydrin at 30℃. After 7 hours reaction, entanselect excess （e.e.） and product yield of （S）- epichlorohydrin reached 99.06% and 17.14%, respectively.
出处
《浙江化工》
CAS
2009年第8期11-16,共6页
Zhejiang Chemical Industry
关键词
手性环氧氯丙烷
黑曲霉
环氧化物水解酶
响应面法
chiral epichlorohydrin
aspergillus niger ZJB-090101
epoxide hydrolase
response surface methodology （RSM）
