摘要
利用离子注入机所产生的低能N+模仿宇宙中低能离子作用于人宫颈癌细胞(HeLa cell),探索其对人类细胞的影响及作用机制。因实验中的低能离子产生和加速要在真空中进行,细胞在离子注入同时将受到真空的影响,为此研究人员利用石蜡油保护细胞以防止注入时的水份蒸发。注入处理完毕后收集细胞,采用傅里叶变换红外光谱法(FTIR)分析真空和低能N+束注入后细胞中大分子的相对含量、构型及其构象变化等方面的信息。结果表明:(1)不同处理后的样品在3 300 cm-1附近吸收谱带存在明显差异。对照样品的特征峰位为3 300 cm-1,而其他样品中除了注入5×1014N+.cm-2外,红外吸收峰均向长波数方向移动,真空2×1015N+.cm-2样品的频移尤为明显至3 420 cm-1处。(2)与对照样品相比较,各处理样品的1 378cm-1处吸收峰峰位均向长波数方向频移。(3)处理样品相对于对照样品而言,2 360 cm-1处吸收峰均向长波数方向移动。该结果说明低能离子注入处理可以引起细胞中核酸、蛋白的含量和构象变化。
In the present paper, low-energy N＋ ions produced by low-energy ionic radiometer were used to simulate low-energy ions in astrospace implanting HeLa cell. Then, the effect and mechanism of low-energy ion on the cell were studied with FTIR spectroscopic analysis. In this study, low-energy ions were produced and accelerated in vacuum, and cells would be affected by vacuum when they were implanted by low-energy ions, so mineral oil was used to protect cells from water evaporation. Cells were collected after being implanted, then the change in the content and constructional form in cellular macromolecule with infra- red spectrometry. Result indicated that the spectroscopic peak position of differently worked cells was obviously different as compared to the control cell （3 300 cm-1 ）. Spectroscopic peak position of all samples except implanting 5 ×10^14N＋·cm^-2 removed to longer wavelength, and the peak position in vacuum of ×10^15N＋·cm^-2 sample moved to 3 420 cm1. In addition, the 1 378 and 2 360 cm-1 spectroscopic peak positions in control cell all moved to longer wavelength in every worked group. In a word, FTIR spectroscopic analysis indicates that low-energy ion implantation could arouse change in nucleic acid or protein in HeLa cell.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2009年第9期2409-2412,共4页
Spectroscopy and Spectral Analysis
基金
国家"十五"科技攻关项目(2001BA302B-03)资助
关键词
低能离子注入
宫颈癌细胞
红外光谱
Low-energy ions implantation
HeLa cell
FTIR spectroscopic analysis
