摘要
目的:探讨左金丸及反左金丸对大肠癌发生、发展的干预作用,及在大肠癌不同时期对甲基转移酶表达的影响.方法:在180只大鼠左肩胛骨侧皮下注射1,2-二甲基酰肼(1,2-DMH)25mg/kg,每周1次,连续注射12wk.随机将大鼠分为3组:左金丸组、反左金丸组和对照组.从第1次注射DMH的第2天开始,左金丸组和反左金丸组每天以中药汤剂1.4g/kg灌胃,对照组灌服同等体积的生理盐水,每日1次,连续34wk.分别在第11、21、34周,每组取部分大鼠脱颈处死,通过病理学观察左金丸和反左金丸对大鼠大肠癌发生发展的干预作用,实时荧光定量PCR法检测不同肿瘤发展时期Dnmt1、Dnmt3a及Dnmt3bmRNA的表达.结果:左金丸和反左金丸均能在肿瘤发生的早期显著抑制癌症的进程,左金丸作用优于反左金丸,但后期(34wk),二者的作用不明显.对照组和中药组Dnmt1、Dnmt3a表达没有明显变化.对照组中Dnmt3b的表达明显高于中药组(4.784vs2.613,P<0.01),且随癌变进程的深入,Dnmt3b表达量增高.结论:左金丸和反左金丸均可以有效阻碍早期大肠癌的发生和发展.Dnmt3b参与了组织恶变的过程,其表达的调控可能是中药干预肿瘤进程的机制之一.
AIM： To investigate the intervention effect of Zuojin Pill and Fanzuojin Pill on the development arid progression of colorectal carcinoma, and examine their effects on the expression of methyltransferase expression in colorectal carcinoma at different stages. METHODS： Rats were given a subcutaneous injection of DMH （25 mg/kg; once a week for 12 weeks） to establish a rat model of colorectal carcinoma. The rats were then randomly divided into Zuojin Pill group （i.g., 1.4 g/kg, once a day）, Vanzuojin Pill group （i.g., 1.4 g/kg, once a day） and control group （i.g., 0.9% NaCl, once a day）. The rats in each group were killed at weeks 11, 21 and 34 after treatment, respectively. The intervention effect of Zuojin Pill and Fanzuojin Pill on the development and progression of colorectal carcinoma was evaluated by pathological examination. The expression of methyltransferase in colorectal carcinoma at different stages was detected by real-time fluorescent quantitative PCR. RESULTS： Zuojin Pill and Fanzuojin Pill could effectively suppress the early development of rat colorectal carcinoma induced by DMH though they had no obvious suppressing effects against the late progression of colorectal carcinoma. In the control group and two treatment groups, the expression levels of Dnmtl and Dnmt3a did not change significantly. However, the expression level of Dnmt3b in the positive control group was significantly higher than those in the two treatment groups （4.784 vs 2.613, P 〈 0.01）. In addition, the expression level of Dnmt3b was gradually elevated as the carcinoma progressed. CONCLUSION： Zuojin Pill and Fanzuojin Pill can effectively hinder the early development of rat colorectal carcinoma induced by DMH. Dnmt3b is involved in the process of malignant transformation of and rat colorectal carcinoma, which may explain the intervention effects of Zuojin Pill and Fanzuojin Pill.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第20期2074-2078,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30400602
No.30672687~~
关键词
左金丸
反左金丸
1
2-二甲基酰肼
大肠癌
甲基转移酶
Zuojin Pill
Fanzuojin Pill
1, 2-dimethyl hydrazide
Colorectal carcinoma
Methyltransferase
