摘要
目的观察归肝经中药对体外人肝癌细胞株HepG2增殖及凋亡的影响，初步探讨归肝经中药抗肝癌作用的趋向性。方法归肝经中药选取莪术、水蛭、丹参，非归肝经中药选取白花蛇舌草为对照；制备提取物后体外干预HepG2细胞。用四甲基偶氮唑盐（MTT）比色法检测各中药提取物对HepG2细胞增殖的抑制作用，并计算半数抑制浓度（IC50）；用细胞计数法、流式细胞仪、吖啶橙／溴化乙锭（AO／EB）双染法分析比较归肝经中药莪术、水蛭、丹参提取物与非归肝经中药白花蛇舌草提取物在IC50药物浓度下对HepG2细胞增殖的影响及诱导凋亡的差异。结果白花蛇舌草提取物IC50药物浓度对应生药量是丹参提取物的11．39倍，水蛭提取物的16．56倍，莪术挥发油的168．67倍。IC50药物浓度作用下，归肝经中药莪术、水蛭、丹参提取物抑制HepG2细胞增殖作用、诱导细胞凋亡率均强于非归肝经中药白花蛇舌草提取物，归肝经中药可阻止HepG2细胞从G0／G1期进入S期，而白花蛇舌草提取物对HepG2细胞周期无明显影响。结论归肝经中药对肝癌HepG2细胞增殖抑制及凋亡诱导作用强于非归肝经中药白花蛇舌草，提示归肝经可能与中药抗肝癌趋向作用有关。
Objective To observe the effect of Chinese material mediea passing through the liver channel （归肝经中药） on inhibition of proliferation and apoptosis of HepG2 cells in vitro and preliminarily investigate the trends of its effects on anti-hepatocellular carcinoma. Methods Zedoary rhizome （莪术）, leech （水蛭） and salvia miltiorrhiza （丹参） passing through the liver channel were selected to compare with hedyotic diffusa （白花蛇舌草） passing through the non-liver channel as the control. The HepG2 cells were treated by extractions from the above drugs. The inhibition of proliferation and 50% inhibitory rate （IC50） were assayed by methyl thiazolyl tetrazolium （MTT） method. The cell growth and apoptosis of HepG2 ceils in vitro which were treated by IC50 drug concentration of all extractions were detected by methods of cell count, flow cytometry and acridine orange/ethidium bromide （AO/EB） staining. The differences of effects were contrasted between zedoary rhizome, leech and salvia miltiorrhiza which pass through liver channel and hedyotic diffusa which passes through non-liver channel. Results The IC50 drug concentration equal to the crude drug content of hedyotis diffusa was 11.39 times more than that of salvia miltiorrhiza, 16.56 times more than that of leech and 168.67 times more than that of zedoray rhizome. The results showed that the reduction rates of cell count and apoptosis induced by zedoray rhizome, leech and salvia extraction which pass through the liver channel were higher than those of hedyotis diffusa extraction which passes through the non-liver channel. The results demonstrated that cell cycle could be prevented from the G0/G1 phase into the S phase by drugs which pass through the liver channel, while the HepG2 cell cycle under treatment of hedyotis diffusa extraction had no significant effect. Conclusion Chinese material medica passing through the liver channel have stronger effect on the inhibition of proliferation and apoptosis of HepG2 cells than the drug passing through the non-liver channel, such as hedyotic diffusa. It is suggested that the liver channel tropism of the Chinese medicinal drugs be related to the tendeney of selective effect on anti-hepatocellular carcinoma.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第4期199-202,F0003,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金项目（30801496）
湖南省自然科学基金项目（06JJ4054）
湖南省教育厅青年基金项目（058036）
关键词
肝癌
凋亡
归经
hepatocellular carcinoma
apoptosis
channel tropism
