摘要
目的测定不同厂家锁阳固精丸中松果菊苷的含量。方法以松果菊苷为测定指标,HPLC法测定锁阳固精丸中松果菊苷含量的色谱条件:色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为乙腈-甲醇-0.2%磷酸(10∶15∶75),检测波长为334 nm,流速为1 ml.min-1。结果不同厂家三个批次的含量分别为0.46、0.55、0.41 mg.g-1。结论定量方法简便、准确、重复性好,可以作为锁阳固精丸的质量控制方法。
Aim To determinea echinacoside in suoyang gujing pills of different pharmaceutical manufactures by HPLC. Methods With echinacoside as the marker, HPLC was used to determine the contents of echinacoside in the condition of chromatographic column Kroma- sil Cls column（250 mm× 4.6 mm,5 μm）. The mobile phase was acetonitrile-methanol -0.2% orthophosphoric acid （10：15：75 ）. The detection wavelength was 334 nm ata flow rate of 1.0 ml ·min -1. Results The contents of three batches were 0.46,0.55,0.41 mg · g- 1 in different pharmaceutical manufactures respectively. Conclusion The method is quick, simple, and repeatable for the determina- tion of echinacoside in suoyang gujing pills. It can be used as quality control in medicinal preparation.
出处
《安徽医药》
CAS
2009年第9期1044-1045,共2页
Anhui Medical and Pharmaceutical Journal
