摘要
采用化学偶联法制备抗ＣＤ３抗Ｂ细胞性白血病独特型双特异性抗体，研究了该双特异性抗体诱导ＬＡＫ细胞的增殖及其细胞毒作用。结果提示，双特异性抗体可显著提高ＬＡＫ细胞与Ｄａｕｄｉ细胞的结合率；促进淋巴细胞的增殖。乳酸脱氢酶试验检测发现，加入双特异性抗体后，ＬＡＫ细胞对Ｄａｕｄｉ细胞的细胞毒性作用显著高于对照组。
The anti CD3×anti B CLL idiotype bispecific antibody(BsAb) was prepared by chemical conjugation. The effect of BsAb on the proliferation and cytotoxicity of LAK cells were studied. The cytotoxic activity of LAK cells was determined by LDH assay. The results showed that BsAb significantly increased the conjugate formation between LAK and Daudi cells and enhanced proliferation of lymphocytes and could enhance effects on the cytotoxicity against Daudi cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1998年第1期40-42,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金
关键词
BsAB
双特异性抗体
制备
细胞毒性
chemical conjugation LAK cell bispecific antibody cytotoxicity
