摘要
试验结果表明,在Cu2+质量浓度为0.096 mg/L水体中生活的文昌鱼酸性磷酸酶(ACP)、碱性磷酸酶(ALP)均不受抑制作用,在Cu2+质量浓度为0.16、0.256、0.356 mg/L水体中生活的文昌鱼的ACP、ALP活性均显著受抑制(P<0.05),随时间延长和质量浓度升高,抑制作用增强。在Zn2+质量浓度达到0.195 mg/L时,文昌鱼的ALP与对照组同期相比受抑制作用显著(P<0.05),随质量浓度增加,受抑制作用增强;ACP在Zn2+质量浓度达到0.39 mg/L时,文昌鱼ACP第10 d影响不显著(P>0.05),第20 d和30 d表现出显著(P<0.05)抑制作用,超过这一质量浓度,受抑制作用增强。在Cd2+水体中生活的文昌鱼ALP在Cd2+质量浓度为0.4 mg/L时,抑制显著(P<0.05),在此质量浓度水体中的文昌鱼ACP在第30 d测定时也表现为抑制显著(P<0.05),随时间延长和质量浓度增加,文昌鱼ACP、ALP活性受抑制作用增强,在最高质量浓度为2.0 mg/L时,至中期已经死亡。
The effects of Cu^2＋ , Zn^2＋ , and Cd^2＋ ions on the activities of acid phosphatase （ACP） and alka-line phosphatase （ALP） were studied in Branchiostoma belcheri in coastal Maoming area in Guangdong province, China. The results showed that the ALP and ACP activities were not restrained at Cu^2＋ concentration of 0. 096 mg/L. However, the ACP and ALP activities were restrained significantly（P〈0.05）at Cu^2＋ concentration of over 0. 096 mg/L, with increase in restraintion with period. The ALP activity was significantly reduced at Zn^2＋ concentration of 0. 195 mg/L （P〈0.05）and reduced more as the concentra- tion increased. At Zn2＋ concentration of 0.39 mg/L, the ACP activity was not affected significantly （P〉 0.05）on the 10 th day, however,affected significantly on the 20 th day and 30 th day（P〈0.05）, with increase in restraintion as concentration increase. The animals had significantly low ALP activity（P〈0.05） when they were exposed to Cd^2＋ concentration of 0.4 mg/L, and had significantly low ACP activity（P〈 0.05） on the 30^th day. When the Cd^2＋ concentration was up to 2.0 mg/L, 100% mortality was observed during the experiment, failing to determination of the phosphatase.
出处
《水产科学》
CAS
北大核心
2009年第9期513-517,共5页
Fisheries Science
基金
广东省自然科学基金资助项目(33981)
