摘要
应用反向间接血凝试验（ＲＰＨＡ）、酶免疫测定（ＥＩＡ）和聚合酶链反应（ＰＣＲ）测定了家禽血清、卵黄和牛乳清中ＨＢＶ标志物及人ＨＢＶ－ＤＮＡ。经ＲＰＨＡ测定发现，ＨＢｓＡｇ阳性率分别为４８／２０５（鸡血清），９／３１（鸭血清），３１／１０７（鸡卵黄）和５／３５（牛乳清）；在ＥＩＡ测定中，ＨＢｓＡｇ的阳性率和滴度均低于ＲＰＨＡ法，而且还发现其他ＨＢＶ标志物（ＨＢｓＡｂ、ＨＢｅＡｇ、ＨＢｅＡｂ和ＨＢｃＡｂ）阳性的样品。对其中阳性样品应用ＰＣＲ测定ＨＢＶ－ＤＮＡ，结果全部阴性。此外，在ＲＰＨＡ测定中，经热处理的样品中５６．９９％由阳性转阴性；ＲＰＨＡ－ＨＩ测定中，人的阳性对照组明显地被抑制，而畜禽的阳性样品几乎均不能被抑制。根据上述结果，提示存在于家禽血清等样品中的ＨＢＶ标志物可能是由非特异性因素和／或其他交叉抗原所引起，而与人ＨＢＶ无关。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). By RPHA the HBsAg positive rates were obtained in chicken serum (48/205), duck serum (9/31), yolk (31/107) and bovine milk whey (5/35). By EIA the HBV markers, such as HBsAb, HBeAg, HBeAb and HBcAb, besides HBsAg were found in above mentioned samples. In addition, the positive rates and their titers of HBsAg obtained by EIA appeared lower than those by RPHA. After the positive samples were treated at 60℃ for one hour, 56.99% of them became negative by RPHA and the agglutination of them did not be specially inhibited by HBsAb. Through the PCR analyses there was no HBV DNA in samples from animals. All these results suggested that the HBV markers in the samples do not relate to HBV and that the HBV markers positive reaction may be produced by non specific factors and/or some cross antigens.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第5期434-436,共3页
Chinese Journal of Veterinary Science
基金
国家教委优秀年轻教师基金
