疏血通对大鼠脑缺血再灌注后TNF-a表达的影响被引量：5

Effect of Shuxuetong TNF-a in rats cerebral ischemia reperfusion

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摘要目的本试验通过观察疏血通对大鼠脑缺血再灌注后TNF-a含量的影响,探讨其保护作用及作用机制。方法Wistor雌、雄性大鼠共42只,随机分为假手术组、盐水对照组及疏血通用药组,对照组及用药组再进一步分为3、6、24h,根据Koizumi线栓法建立大鼠左侧大脑中动脉缺血再灌注模型(MCAO)。用免疫组化检测血清和脑组织匀浆肿瘤坏死因子TNF-a含量。结果TNF-a含量假手术组几乎无TNF-a的表达,盐水组TNF-a表达3h开始升高,于6h达到高峰并逐渐下降。用药组较盐水组TNF-a含量明显减少P<0.01。结论疏血通对大鼠脑缺血再灌注损伤具有保护作用,其机制可能与减少TNF-a含量有关。 Objective Shuxuetong was evaluated its protective effects through observing expression of TNF-a. Methods 42 wistor rats were randomly divided into 3 grout,s, sham operation group, saline control group and Shuxuetong treatment group. Saline control group and Shuxuetong treatment group were divided into 3 h, 6 h,24 h. The left middle cerebral artery ischemia-reperfusion injury model （MCAO） was made by Koizumi suture-occluded method. The content of tumor necrosis factor-a （TNF-a） in serum and brain, were evaluated with immunohistochemical method . Results The expression of TNF-a of cerebral ischemia-reperfusion： There was almost no expression of TNF-a in sham operation group. The expression of TNF-a in saline control group increased in 3 h and reached peak in 6 hours and then gradually decreased. Compared with that in saline control group, the expression of TNF-a in Shuxuetong treatment group obviously reduced （ P 〈 0. 05 ）. Conclusion Shuxuetong could reduce the expression of TNF-a and protect the brain.

作者姜炎田志强牛延良

机构地区郑州大学第五附属医院神经内科

出处《中国实用医药》 2009年第27期26-27,共2页 China Practical Medicine

关键词脑缺血再灌注 TNF-A 疏血通 Cerebral ischemia-reperfusion Shuxuetong TNF-a

分类号 R285.5 [医药卫生—中药学]

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参考文献9

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