摘要
马铃薯试管苗在全价、60%磷素和30%磷素的MS培养基上培养,第20 d、40 d时测定生理指标。结果表明,低磷胁迫对马铃薯试管苗新叶和侧根发生以及根生长具有促进作用,且地上部和地下部鲜重小幅增加。30%低磷培养环境下,试管苗的细胞膜透性值、可溶性蛋白质和可溶性糖含量均较对照明显增加,而叶绿素含量则明显降低,差异达到极显著或显著水平。60%低磷处理下,这些指标的变化与对照基本相同,差异不显著,表明含60%P元素的MS培养基适宜马铃薯试管苗生长。
Potato plantlets in vitro were cultured on MS medium with full phosphorus level, 60% phosphorus level and 30 % phosphorus level. Physiological indexes on 20 d and 40 d were determined and the results showed that the ability of new leaves and lateral root generation was promoted under low-p stress, which could also improve the root growth, increase the fresh weight of aboveground and underground slightly. Under the culture environment with 30 % phosphorus, the relative permeability of cell membrane, soluble protein content and soluble sugar content increased and chlorophyll content decreased significantly compared with the control, and the significant difference was at the level of 5 % or 1%. Under the treatment with 60 % phosphorus, the changes of physiological indexes were the same as the control, and there was no obvious difference. The results demonstrated that it is suitable for potato plantlets in vitro growth on MS medium with 60% phosphorus.
出处
《干旱地区农业研究》
CSCD
北大核心
2009年第5期183-187,共5页
Agricultural Research in the Arid Areas
基金
国家科技支撑计划项目(2006BAD21B05)
农业部行业专项项目(nyhyzx-07-006-3)
甘肃省援藏项目(0708JKCA063)
关键词
马铃薯
试管苗
低磷胁迫
生理指标
potato
plantlet in Vitro
low-phosphorus stress
physiological index
