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异源Oct-4基因启动子在猪、兔和小鼠早期胚胎中的时空表达活性 被引量：3

Spatio-temporal Expression of Heterologous Oct-4 Promoter in Preimplantation Embryos of Pig, Rabbit and Mouse
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摘要 Oct-4是一种哺乳动物早期胚胎中特异表达的转录因子,它与细胞多能性的维持有关.异源Oct-4基因在早期胚胎中的表达模式尚不明确.构建了一个以完整的牛Oct-4调控区指导GFP表达的转基因结构pOct-4(p)-GFP,通过单精子注射的方法将其导入猪、兔和小鼠的受精卵中,分析其在胚胎发育过程中的表达情况.结果显示:牛Oct-4启动子驱动的GFP基因在3个物种的2-细胞胚胎就已经开始表达,在囊胚期表达加强且只特异表达于内细胞团中,而不表达于滋养层.研究表明:牛的Oct-4启动子在其他物种中也具有表达活性,异源性Oct-4启动子在不同物种的早期胚胎中具有相似的表达模式. The transcription factor Oct-4 is expressed specifically in mammalian preimplantation embryos and its function is related to the maintenance of embryonic stem cell pluripotency. The functional role of the heterogenous expression of Oct-4 remains unclear however. A GFP reporter construct, pOct-4（p）-GFP was generated, containing the upstream regulatory regions of bovine Oct-4 gene and its expression pattern was evaluated in the developing embryos of mouse, pig and rabbit following intracytoplasmic sperm injection. GFP fluorescence was visible early at the 2-cell stage and then became stronger in the blastocysts of all three species. However, the distribution of the GFP signals was restricted to the cells of inner cell mass and no fluorescence was detectable in trophectoderm cells. These results suggest that the bovine Oct-4 promoter is functional and that its embryonic expression activity is similar in different mammalian species.
作者 张颖 史芳瑜 高绍荣 林爱星
机构地区 中国农业大学生物学院 北京生命科学研究所
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2009年第9期1180-1185,共6页 Progress In Biochemistry and Biophysics
基金 国家自然科学基金资助项目(30570947) 国家高技术研究发展计划(863)部分资助项目(AA02Z113)~~
关键词 OCT-4 早期胚胎 猪 兔 小鼠 Oct-4, preimplantation embryos, pig, rabbit, mouse
分类号 Q7 [生物学—分子生物学]
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参考文献23

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同被引文献63

  • 1张梅英,李华,董婉维,杨葳,秦英,汪瑛,周生来,于洋,王惟,王太一,王禄增.稳定整合pTet-on载体小鼠胚胎干细胞株的建立[J].中国实验动物学报,2006,14(3):161-166. 被引量：2
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  • 4Maherali N. Sridharan R. Xie W. et al, Directly repro?grammed fibroblasts show global epigenetic remode?ling and widespread tissue contribution[]]' Cell Stem Cell.2007 .1(1) ,55-70.
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  • 6Takahashi K.Tanabe K.Ohnuki M.et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors[J]. Cell.2007 .131(5) ,861-872.
  • 7Wernig Mv Meissner As Foreman Rver al. In vitro re?programming of fibroblasts into a pluripotent ES-cell?like state[J]. Nature, 2007 .448(7151) ,318-324.
  • 8Niwa H. Miyazaki]. Smith A G. Quantitative expres?sion of Oct-3/4 defines differentiation. dedifferentia?tion or self-renewal of ES cells[J]. Nat Genet,2000. 24(4) ,372-376.
  • 9Pesce Mv Marin G MvPhilipsen Sv et al. Binding of Spl and Sp3 transcription factors to the Oct-4 gene pro?moter[J]. Cell Mol Bioi (Noisy-Ie-Grand. France). 1999.45(5),709-716.
  • 10Schoorlemmer r. Van Puijenbroek A. Van Den Ei- jnden M. et al. Characterization of a negative retinoic acid response element in the murine Oct4 promoter[J]. Mol Cell Biol.1994 .14(2),1122-1136.

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生物化学与生物物理进展
2009年 第9期

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