摘要
研究发现门多萨假单胞菌NK-01在菌体内积累中长链聚羟基脂肪酸酯(PHAMCL)的同时也能够合成褐藻寡糖分泌到发酵液中,其产量与培养基的碳氮比有关,高碳氮比有利于褐藻寡糖的合成。本研究利用紫外-可见分光光度法、傅立叶红外光谱分析、1H和13C核磁共振对褐藻寡糖的结构进行了分析鉴定,发现褐藻寡糖的结构是由β-D-甘露糖醛酸、α-L-古洛糖醛酸通过β-(1→4)/α-(1→4)键连接而成的无支化线性多糖,并且在单体的2位或3位羟基上部分乙酰化。凝胶渗透色谱(GPC)对分子量的测定结果为2054。
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly （3-hydroxyalkanoates）, it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, ^1H and ^13C of Nuclear Magnetic Resonance, and found that it was compounded in line from β-D-mannuronic acids and α-L-gluronic acids via β-（1→4）/ α-（1→4） bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
出处
《生物工程学报》
CAS
CSCD
北大核心
2009年第9期1366-1370,共5页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划(863计划)(No.2007AA06Z323)
天津市基础研究重点项目(No.09JCZDJC18400)资助~~
关键词
门多萨假单胞菌
发酵
褐藻寡糖
结构鉴定
Pseudomonas mendocina, fermentation, alginate oligosaccharides, structure characterization
