摘要
利用盆栽实验，对幼苗期灰绿和黄绿两个生态型羊草用NaCl和Na2CO3进行胁迫后，采用紫外分光光度法对可溶性蛋白含量进行了测定．定量分析结果表明，在不同浓度NaCl和Na2CO3胁迫条件下，灰绿型和黄绿型羊草可溶性蛋白质含量分别是对照的2．52～3．52倍，4.87-10．32倍，1．60～3．58倍和1．95～6．67倍．灰绿型和黄绿型羊草可溶性蛋白质含量在NaCl浓度为100mmol／L和25mmol／L，Na2C03浓度为100mmol／L和50mmol／L时达到最高值．由此表明，灰绿型和黄绿型羊草具有一定的耐盐碱能力，且灰绿型羊草的耐盐碱能力高于黄绿型羊．
At the stage of seedlings,the which are planted with sand culture an have been determined with ultraviolet tents of soluble protein of tillers of th times,4.87~10.32 times, 1.60~3.58 Na2CO3 salt-alkali stresses gradients th of tillers of grey green and yellow green ecotype L. chinensis, d stressed by NaCl and Na2CO3 salt-alkali stresses gradients, spectrophotometric method. Based on data analysis, the contents of soluble protein of tillers of the grey-green and the yellow-green ecotype were 2. 52 ~ 3. 52 times and 1.94~6.67 times bigger under different NaC1 and an those under no NaC1 and Na2CO3 salt alkali stress,respectively. The contents of soluble protein of tillers of the grey green and the yellow-green ecotype reached the biggest value under NaCI concentration of 100 mmol/L and 25 mmol/L, Na2CO3, concentration of 100 mmol/L and 50 mmol/L, respectively. L. chinensis adapts to severe conditions by means of strain tolerance. In results, there is some salt-alkali tolerance of two experimental populations,but salt-alkali tolerance of grey-green ecotype is stronger than that of yellow-green ecotype. It shows the most important meaning in breeding variety of resisting salt-alkali, harnessing salivation steppe and production.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期94-96,共3页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(30770397
30470272)
辽宁大学青年科学基金资助项目
关键词
羊草
盐碱胁迫
可溶性蛋白质
紫外分光光度法
L. chinensis
salt-alkali stress
soluble protein
ultraviolet spectrophotometric method
