摘要
目的探讨雷公藤内酯醇对重症急性胰腺炎（SAP）肺损伤大鼠的保护作用及其可能机制。方法将36只雄性Wistar大鼠随机分为假手术组（sham组）、模型组和雷公藤内酯醇处理组（Tri组）3组，每组6只。sham组仅行剖腹术；Tri组和模型组采用牛磺胆酸钠胰胆管逆行注射法复制SAP肺损伤模型，然后分别腹腔注射雷公藤内酯醇0．2mg／kg或等量生理盐水。制模后6h处死动物，取肺组织测定肺组织肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）的mRNA表达、蛋白含量及核转录因子-κB（NF-κB）活性。结果与sham组比较，模型组肺组织TNF-αmRNA与IL-1βmRNA表达及蛋白含量、NF-κB活性均显著升高；与模型组比较，Tri组上述指标均明显降低，差异均有统计学意义（P均〈0．01）。结论雷公藤内酯醇对SAP肺损伤大鼠具有一定保护作用，与抑制NF-κB活性进而下调TNF-α、IL-1β等炎症因子表达有关。
Objective To investigate the protective effects of triptolide on severe acute panereatitis （SAP）-associated lung injury in rats and assess its possible mechanism. Methods Thirty-six male Wistar rats 250 300 g in weight were randomly divided into three groups （n= 6 each）： sham, model and triptolide （Tri） groups. Sham group only underwent laparotomy. Immediately after undergoing retrograde injection of sodium taurocholate into pancreatic duct, Tri and model groups received intra-peritoneal injection of triptolide 0.2 mg/kg or equal amount of normal saline, respectively. Six hours after sodium taurocholate injection, the rats were sacrificed. The lungs were removed for the determinations of the mRNA and protein expression levels of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β）, and nuclear factor--κB （NF--κB） activity. Results Compared with sham group, model group showed a significant increase in the mRNA and protein expression levels of TNF-α and IL-1β, and NF--κB activity in the lung tissues （all P〈0. 01）. In Tri group, all of these indexes were significantly lower than those of model group （all P 〈0. 01）. Conclusion The treatment with triptolide has protective effects against SAP-associated lung injury in rats. The underlying mechanism is probably via a down-regulation in lung inflammatory cytokines, such as TNF-α and IL-1β, through the inhibition of NF--κB activity.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第5期296-298,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
基金项目：浙江省温州市科技计划项目（Y2005A144）
