摘要
目的建立诃予提取物中鞣花酸含量的高效液相色谱（HPLC）测定方法。方法色谱条件Shim—pack CLC—ODS色谱柱（150mm×6mm，5μm），柱温30℃，用CH3OH—EtOAc—KH2PO4／H3PO4（0．05mol／L）（体积比30：2：68）作为流动相，检测波长为254nm。结果鞣花酸在0．268～5．36μg范围内与峰面积呈良好的线性关系。平均加样回收率为98．42％，RSD为1．13％（n=5）。结论该方法快速、简便、准确，可用于诃子中鞣花酸的含量测定。
Objective To develop an HPLC method for the determination of ellagic acid in the extract of Terminalia . Methods HPLC separation was carried out in an Shim -pack CLC -ODS columm （150 mm×6 mm,5 μm）with the mobile phase consisted of methanol ethyl acetate, 0.05 mol/L KH2PO4 -H3 PO4 （34： 2： 64） at a flow rate of 1.0 ml/min. The detection wavelength was 254 nm. Results The linear range was 0. 268 - 5.36 μg/ml for ellagic acid. The average recovery was 98.42% , and RSD was 1.13% （ n =5）, Conclusion This method is rapid, accurate and reproducible for determination of ellagic acid in the extract of Terminalia.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第9期2178-2179,共2页
Lishizhen Medicine and Materia Medica Research
基金
云南省教育厅科学研究基金项目资助(No.2007B148M)
