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溶菌酶和乳铁蛋白在慢性鼻窦炎钩突粘膜中的表达 被引量:7

Lysozyme and lactoferrin in human uncinate process mucosa during chronic sinusitis
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摘要 目的了解钩突在局部防御慢性炎症过程中的作用。方法采用免疫组化ABC法,检测溶菌酶(lysozyme,LZ)和乳铁蛋白(lactoferin,LF)在17侧健康和70侧慢性鼻窦炎患者(分为单纯炎症组和合并鼻息肉组)钩突粘膜中的表达情况。结果①LZ在单纯炎症组钩突粘膜杯状细胞中表达较健康对照组加强(P<0.05);LF在健康和慢性鼻窦炎钩突杯状细胞中常为阴性表达;②LZ在健康和慢性鼻窦炎钩突粘膜腺体的浆液性和粘液性细胞中均有表达;LF只在浆液性腺细胞胞浆中表达。合并鼻息肉组钩突粘膜腺细胞中LZ和LF的表达增强,其LZ的表达与健康对照组和单纯炎症组相比差异均有显著性(P<0.01,P<0.05),LF的表达与健康对照组比较差异有非常显著性(P<0.01);③钩突粘膜上皮细胞对LZ和LF多为阴性表达。结论钩突粘膜中的杯状细胞和混合腺对LZ和LF的分泌起重要作用,可能参与构成局部免疫防御系统。 Objective To study the local protective role of lysozyme(LZ) and lactoferrin(LF) in the uncinate process mucosa during chronic sinusitis. Methods Expression of LZ and LF was determined in 17 samples from normal subjects and 70 samples from chronic sinusitis patients with ABC immunohistochemical method. According to the presence or absence of nasal polyps, patients were divided into two groups. Results Serous cells of submucosal glands displayed a strongly positive staining reaction to both LZ and LF in the normal uncinate process mucosa and mucosa from patients with chronic sinusitis. A positive though weak staining for LZ could also be found frequently within mucous cells of submucosal mixed glands and occasionally within goblet cells. In the mucosa from patients without nasal polyps,the staining reaction to LZ appeared to be intensified in goblet cells when compared with normal controls (P<0.05). In patients with nasal polyps,the staining reaction to LZ appeared to be intensified in submucosal glands when compared with normal controls (P<0.01) and patients without nasal polyps (P<0.05). For LF,the staining reaction from patients with nasal polyps was stronger than that in normal controls (P<0.01).The epithial cells stained negatively for LZ and LF. Conclusions It suggests that the observed increase in LZ and LF secreting activity of goblet cells and submucosal mixed glands may play a part role in the defense mechanism of uncinate process mucosa during the course of chronic sinusitis.
出处 《中华耳鼻咽喉科杂志》 CSCD 1998年第4期219-221,共3页 Chinese Journal of Otorhinolaryngology
关键词 鼻窦炎 溶菌酶 乳铁蛋白 免疫组化 Sinusitis Muramidase Lactoferrin Immunohistochemistry
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参考文献1

  • 1姚笠,国外医学.免疫学分册,1996年,19卷,317页

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