摘要
目的探讨热休克蛋白（ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎｓ，ＨＳＰｓ）在喉癌组织及其正常喉粘膜中的表达。方法采用狭缝杂交（Ｓｌｏｔｂｌｏｔａｎａｌｙｓｉｓ）技术对２１例喉癌组织及其正常喉粘膜的ＨＳＰ９０、ＨＳＰ７０和ＨＳＰ２７ｍＲＮＡ表达水平进行检测。结果①ＨＳＰ９０α和ＨＳＰ７０在喉癌组织中呈过度表达，表达量是自身正常喉粘膜的５倍以上。②ＨＳＰ２７和ＨＳＰ９０β在喉癌组织及正常喉粘膜中呈低水平表达，其表达量在两种组织中差异无显著性。结论ＨＳＰ７０和ＨＳＰ９０α在喉癌的致病过程中起着重要的作用。弄清它们的作用机理，将为ＨＳＰｓ用于喉癌的生物治疗提供理论依据。
Objective To determine the expression of HSPs(HSP90α, HSP90β , HSP70, HSP27) in laryngeal carcinoma and normal laryngeal mucosa. Methods Total RNA were extracted from 21 laryngeal cancer tissue samples and the normal mucosa of the corresponding larynx. The expression of HSPs mRNA was examined by Slot blot analysis. Results ①HSP90 α and HSP70 were selectively overexpressed 5 times more in laryngeal carcinoma than in normal mucosa of larynx. ②HSP90 β and HSP27 were constitutively expressed at low level in these two groups. There was no significant difference between the levels of expression of HSP90 β and HSP27 in laryngeal carcinoma and normal controls. Conclusion The results that HSP90 α and HSP 70 mRNA were selectively overexpressed in laryngeal carcinoma suggested that HSP90α and HSP70 might play a specific role in the pathogenesis of laryngeal cancer.To clarify the mechanism of HSPs action could help to provide theorical basis for the biological therapy of laryngeal cancer.
出处
《中华耳鼻咽喉科杂志》
CSCD
1998年第4期232-233,共2页
Chinese Journal of Otorhinolaryngology
基金
卫生部医学科学研究基金
关键词
喉肿瘤
热休克蛋白
基因表达
Laryngeal neoplasms Heatshock proteins Gene expression
