摘要
目的探讨人疱疹病毒６型（ＨＨＶ６）对ＥＢ病毒（ＥＢＶ）复制的影响。方法用ＨＨＶ６感染Ｒａｊｉ细胞和ＥＢＶ转化的淋巴细胞（ＬＣＬ），并用抗ＨＨＶ６单克隆抗体（ＭＡｂ）作免疫萤光分析（ＩＦＡ）确定ＨＨＶ６已感染这二种细胞。用抗ＥＢＶ人血清作ＩＦＡ观察ＥＢＶ抗原表达。结果ＨＨＶ６感染Ｒａｊｉ细胞后可见细胞病变效应（ＣＰＥ）；而ＬＣＬ未见ＣＰＥ。这二种细胞株用抗ＨＨＶ６的ＭＡｂ作ＩＦＡ均检出ＨＨＶ６，而对照组未检出ＨＨＶ６；用抗ＥＢＶ人血清作ＩＦＡ，感染ＨＨＶ６的二种细胞中均检出ＥＢＶ抗原。结论ＨＨＶ６促进了ＥＢＶ抗原表达，并提示在鼻咽癌的发生中ＨＨＶ６和ＥＢＶ可能起协同致病作用。
Objective To examine the effect of human herpesvirus 6(HHV6) on the replication of EpsteinBarr virus (EBV) in cell lines. Methods Both EBVinfected Raji cells and EBV transformed lymphoblastoid cell lines (LCL) were infected with HHV6. Immunofluoresence assay (IFA) with monoclonal antibodies (MAb) against HHV6 was applied to confirm the infection of HHV6 in the two cell lines. Expression of EBV antigen was examined by IFA using human antiEBV serum. Results Following HHV6 infection, cytopathic effects (CPE) were observed in Raji cells but not in LCL. HHV6 were detected in both cell lines by IFA with antiHHV6 MAb, but not in controls. EBV antigens were detected by IFA with human serum against EBV in both HHV6 infected cell lines. Conclusion Data in this study suggest that HHV6 promotes the expression of EBV antigen and may contribute to the pathogenesis of nasopharyngeal carcinoma in cooperation with EBV.
出处
《中华耳鼻咽喉科杂志》
CSCD
1998年第4期235-236,共2页
Chinese Journal of Otorhinolaryngology
关键词
鼻咽肿瘤
疱疹病毒
病毒复制
Nasopharyngeal neoplasms Herpesvirus 6, human Herpesvirus 4, human Virus replication
