摘要
目的：建立测定中药和保健食品中低聚果糖（包括蔗果三糖、蔗果四糖、蔗果五糖3种成分）的高效液相含量测定方法。方法：色谱柱Agilent zorbaxNH2（4．6min×250mm，5μm），柱温25℃；流动相水-乙腈（32：68），流速1．2mL·min^-1；视差折光检测器，内部温度35℃。结果：蔗果三糖、蔗果四糖、蔗果五糖的线性范围分别为2．26～22．6μg，2．16-21．6μg，2．10-21．0μg；加样回收率（n=5）分别为96．32％，96．98％，95．67％。结论：本方法简便、灵敏、准确，可用于含低聚果糖中药和保健食品中以上3种成分的含量测定和产品质量控制。
Objective.. To develop an RP HPLC-RID method for determination of fructooligosaccharides （including kestose, nystose, Fructofranosylnystose） in chinese medicine and functional foods. Methods： An Agilent zorbax NH2 column （4. 6 min×250mm, 5μm）was applied; a mobile phase composing of acetonitrile and water（68：32） was used, and the flow rate was 1.2 mL · min^-1 ; A RID detector was applied, and the inner temperature was set at 35℃. Results：The results were obtained that quantitative line range of three fructooligosaecharides was 2.26-22. 6μg,2.16-21.6μg,and 2.10-21.0μg respectively, and the average recovery （n=5） was 96.32%,96.98%,and 95.67%, respectively. Conclusion：A specific, sensitive, simple and reproducible RP- HPLC-RID method was established for the determination of the three ingredients in chinese medicine and functional foods that claimed containing fructooligosaccharides.
出处
《亚太传统医药》
2009年第9期20-22,共3页
Asia-Pacific Traditional Medicine
