摘要
建立用中性蛋白酶水解蓝圆鲹的最佳工艺条件。以水解度为优化指标,利用单因素试验考察酶解温度、pH值、反应时间等因素对鱼蛋白水解的影响,利用中心组合试验方法优化酶的添加量和蛋白质浓度。结果表明,加酶量3260U/g蛋白、蛋白质浓度1.47%、水解温度50℃、水解pH值7.0、水解时间10h,水解液中的氨基酸态氮含量达1.09mg/mL,水解度为46.20%,总氮回收率为92.40%,挥发性盐基氮含量为0.047mg/mL,氨基酸态氮、水解度和总氮回收率的RSD值都小于3%。该水解条件水解度较高,总氮回收率较高,工艺稳定性好。
The aim of this paper is to develop a process for hydrolysis Decapterus maruadsi with neutral protease. The effects of the main parameters including hydrolysis temperature, pH value and hydrolysis time were investigated with single factor experiment, and dosage of enzyme, protein concentration were investigated with central combination experiment. The optimized conditions were：concentration of neutral protease was 3260U/g protein,protein concentration was 1.47% , hydrolysis temperature was 50℃ ,hydrolysis pH value was 7.0,and hydrolysis time was 10 hours. Under the conditions,amino acid nitrogen concentration attained 1.09mg/ml, hydrolysis degree attained 46.20% , recovery of the total nitrogen attained 92.40% and total volatile basic nitrogen reached 0. 047mg/ml. RSD value of amino acid nitrogen concentration, hydrolysis degree and recovery of the total nitrogen was lower than 3%. In short, under these conditions, degree of hydrolysis and the recovery of total nitrogen were high, and the process was stable.
出处
《中国酿造》
CAS
北大核心
2009年第9期114-117,共4页
China Brewing
基金
省属高校资助项目(2007F5075)
关键词
蓝圆鲹
中性蛋白酶
水解
优化
Decapterus maruadsi
neutral protease
hydrolysis
optimization
