摘要
用含不同剂量金霉素和喹乙醇的猪粪饲喂锦鲤,分别测定摄食10、20和30d时肌肉组织中抗生素含量、体表和消化道微生物数量,试验结束时对肝脏中超氧化物歧化酶(superoxide dismutase,SOD)活性和脂质过氧化物丙二醛(malondialdehyde,MDA)含量进行了测定。结果表明,猪粪中金霉素和喹乙醇在养殖水体中溶出存在较大差异,从试验第1d到结束养殖水体中喹乙醇浓度Ⅲ和Ⅳ组分别为0.05～0.32mg·kg-1和0.12～0.70mg·kg-1,锦鲤肌肉组织中喹乙醇浓度Ⅲ和Ⅳ组在试验的10、20和30d分别为1.87、5.25、3.83mg·kg-1和2.96、4.33、4.23mg·kg-1。没有在水环境和锦鲤体内检测到金霉素。锦鲤体表和消化道微生物随着加样次数增加,数量也急剧上升。猪粪中的成分能显著降低SOD酶活性,增加MDA含量,而加入金霉素和喹乙醇也未能使锦鲤肝脏SOD活性和MDA含量恢复到机体正常水平。
Koi Carp Cyprinus carpio was fed with swine manure containing different concentrations of chlortetracycline or olaquindox for 30 days, respectively. The number of micro-organisms of body surface and digestive tract, concentrations of antibiotics in the tissue, superoxide dismutase （SOD） and malondialdehyde （MDA） in liver were determined during the experiment. The results showed that the number of microorganisms of carp surface and intestinal tract increased with the usage amount of pig manure. Olaquindox residues were detected in the aquatic water with the concentration from 0.05to 0.70 mg·kg^-1 and in edible tissue with the level ranged from 1.87 mg·kg^-1 to 5.25mg·kg^-1 while chlortetracycline residues were not found in Koi Carp tissue and their aquatic water in all experimental groups. The pig manure and two antibiotics significantly affect the SOD activity and MDA content of the fish liver and the effect depends on the exposure amount of antibiotics.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第9期1800-1805,共6页
Journal of Agro-Environment Science
基金
江苏省农科院基金(0610801-3)
江苏省农业科技自主创新资金(cx(08)102)
环保公益性行业科研专项(200809092A)
关键词
金霉素
喹乙醇
锦鲤
生态毒理效应
chlortetracycline
olaquindox
Koi Carp
ecotoxicological effects
