期刊文献+

利用微悬臂梁研究聚N-异丙基丙烯酰胺在金表面的自组装 被引量:3

Detection of the self-assembly of poly-(N-isopropylacrylamide) on gold based on microcantilever sensor
原文传递
导出
摘要 利用微悬臂梁传感技术研究了巯基化的聚N-异丙基丙烯酰胺(HS-PNIPAM)在微悬臂梁金面上的自组装过程.由于大分子的构象变化改变了分子间的相互作用,导致微悬臂梁的表面应力改变,使微悬臂梁发生弯曲.通过光学方法实时读出微悬臂梁的弯曲信号,得到HS-PNIPAM的自组装动力学曲线.通过对不同分子量HS-PNIPAM的实验结果分析表明:HS-PNIPAM在自组装过程中存在三个阶段,分别对应不同的分子构象.第一阶段为物理吸附阶段,第二、三阶段为伴随着分子构象变化的化学吸附阶段.吸附曲线符合Langmuir等温吸附.分析结果还显示HS-PNIPAM的表面吸附速率κ远小于小分子的吸附速率,并与分子量成负指数关系;HS-PNIPAM的自组装时间远大于小分子的自组装时间,并与分子量成正比;底物表面应力的改变与HS-PNIPAM的分子量成线性关系. A microcantilever sensor platform is used for detecting the self-assembly of poly-(N-isopropylacrylamide) (HS-PNIPAM) on gold surface. The change of the interaction between molecules caused by conformation transition will change the surface stress of the microcantilever which causes its bending. The kinetic curves of self-assembly can be obtained by real-time monitoring the deflection of the microcantilever using the optical lever read-out technique. HS-PNIPAMs of different molecular weight were used to study the self-assembly process, and the results showed that the kinetic carves can be divided into three stages corresponding to different conformations, respectively. The first stage cor responds to physical adsorption of HS-PNIPAM on gold-coated side. The second and third stages correspond to chemical adsorption on gold-coated side with conformation transition. The kinetic curves fit Langmuir adsorption isotherm well. The results also show that the reaction rate κ of HS-PNIPAM is far less than that of small molecules and decreases exponentially with the molecular weight; while the time of HS-PNIPAM' s self-assembly is far greater than that of small molecules and proportional to the molecular weight. The change of the surface stress is linear to the molecular weight of HS-PNIPAM.
出处 《物理学报》 SCIE EI CAS CSCD 北大核心 2009年第9期6122-6127,共6页 Acta Physica Sinica
基金 国家自然科学基金(批准号:10732080,20674078和10872191)资助的课题~~
关键词 微悬臂梁 聚N-异丙基丙烯酰胺 自组装 构象转变 microcantilever poly(N-isopropylacrylamide) self-assembly conformation transition
  • 相关文献

参考文献30

  • 1Butt H J 1996 J. Colloid Interf. Sci. 180 251.
  • 2Sadera J E 2001 J.Appl. Phys. 89 8911.
  • 3Berger R, Delamarche E, Lang H P, Gerber C, Gimzewski J K, Meyer E,Guntherodt H J 1997 Science 276 2021.
  • 4Moulin A M, O'Shea S J, Badley R A,Doyle P, Welland M E 1999 Langmuir 15 8776.
  • 5Hansen K M, Ji H F, Wu G H, Datar R, Cote R, Majumdar A, Thundat T 2001 Anal. Chem. 73 1567.
  • 6Wu G H, Datar R H, Hansen K M, Thundat T, Cote R J, Majumdar A 2001 Nat. Biotechnol. 19 856.
  • 7李凯,刘红,张青川,侯毅,张广照,伍小平.利用微悬臂梁表面应力研究聚N-异丙基丙烯酰胺分子的构象转变[J].物理学报,2006,55(8):4111-4116. 被引量:11
  • 8Evans S D, Urankar E, Ulman A, Ferris N 1991 J. Am. Chem. Soc. 113 4121.
  • 9Whitesides G M, Laibinis P E 1990 Langmuir 6 87.
  • 10Nuzzo R G, Dubois L H, Allara D L 1990 J.Am. Chem. Soc. 112 558.

二级参考文献33

共引文献27

同被引文献34

  • 1曹艳红,孟宪清,冯杰,惠洋,孙殿军.检测T-2毒素的直接竞争性酶联免疫吸附测定法的研究[J].中国人兽共患病学报,2006,22(2):132-135. 被引量:8
  • 2Patil S J, Adhikari A, Baghini M S, et al. An Ultra-Sensitive Piezo- resistive Polymer Nano-Composite Microeantilever Platform for Humidity and Soil Moisture Detection [J]. Sensors and Actuators B ,2014(203) : 165-173.
  • 3Rajavelu M, Sivakumar D, Daniel R J, et al. Perforated Dia- phragms Employed Piezoresistive MEMS Pressure Sensor for Sen- sitivity Enhancement in Gas Flow Measurement IJl. Flow Mea- surement and Instrumentation, 2014( 35 ) : 63-75.
  • 4Sang Shengbo, Zhao Yuan, Zhang Wendong, et al. Surface Stress- Based Biosensors [J]. Biosensors and Bioeleetronies, 2014 (51) : 124-135.
  • 5Thuau D, Ayela C, Poulin P, et al. Highly Piezoresistive Hybrid MEMS Sensors [J]. Sensors and Actuators A, 2014 (209) : 161- 168.
  • 6Yang S M, Chang C. A Piezoresistive Bridge-Microcantilever Bio- sensor by CMOS Process for Surface Stress Measurement[J]. Sen- sors and Actuators B,2010(145) :405-410.
  • 7Pandya H J, Roy R, Chen W J, et al. Accurate Characterization of Benign and Cancerous Breast Tissues: Aspecific Patient Studies Using Piezoresistive Microcantilevers[J]. Biosensors and Bioelec- tronics, 2015 (63) : 414-424.
  • 8Garcia-Romeo D, Pellejero I, Urbiztondo M A, et al. Portable Low- Power Electronic Interface for Explosive Detection Using Micro- cantilevers [ J ]. Sensors and Actuators B, 2014 (200) : 31-38.
  • 9Sang S B, Zhao Y, Zhang W D, et al. Surface Stress-Based Biosen-sors [ J ]. Biosensors and Bioelectronics, 2014(51 ) : 124-135.
  • 10Zhang Q, Ruan W Z, Wang H, et al. A Self-Bended Piezoresistive Microcantilever Flow Sensor for Low Flow Rate Measurement [J]. Sensors and Actuators A, 2010(158) :273-279.

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部