摘要
目的：探讨幽门螺杆菌（Ｈｐ）感染与ｒａｓ原癌基因产物ｐ２１（ｒａｓｐ２１）蛋白和ｐ５３癌基因产物突变型ｐ５３蛋白表达的关系。方法：应用免疫组织化学方法，检测慢性浅表性胃炎（ＣＳＧ）３０例、胃粘膜不全结肠型肠化生（ＩＣＭ）２５例、异型增生２９例、胃癌３７例的活检标本。结果：ＩＣＭ组、异型增生组和胃癌组的Ｈｐ感染率均明显高于ＣＳＧ组（Ｐ＜０．０５）。ｒａｓｐ２１及突变型ｐ５３蛋白的阳性表达率在ＣＳＧ组均为０，而在异型增生组和ＩＣＭ组的表达均明显低于胃癌组（４５％和３８％、３２％和３２％对６０％和７０％），Ｐ＜０．０５，Ｐ＜０．０１；其中胃癌、异型增生及ＩＣＭ组中的Ｈｐ阳性组的ｒａｓｐ２１及突变型ｐ５３蛋白表达阳性率均明显高于Ｈｐ阴性组（Ｐ＜０．０５或Ｐ＜０．０１）。经根除Ｈｐ治疗后，Ｈｐ根除者的ｒａｓｐ２１及突变型ｐ５３蛋白表达由阳性转为阴性的例数比Ｈｐ未根除者多（Ｐ＜０．０５，Ｐ＜０．０１）。结论：Ｈｐ感染可能参与了ｒａｓ癌基因的激活和ｐ５３抑癌基因的突变，这可能是其致癌机制之一。
Objective:To investigate the relationship between Hp infection and expression of ras protooncogene product p21 protein(ras p21)or p53 cancer suppressive gene product mutant p53 protein (p53).Methods:30 of chronic superficial gastritis(CSG),25 of the intestinal metaplasia with colonic type(ICM),29 of dysplasia(Dys),37 of gastric cancer(GC) were examined with biopsy samples by immunohistochemical method.Results:Hp positive rates in ICM,Dys and GC groups were obviously higher than that in CSG group(P<005)Positive expression rates of both ras p21 and p53 were zero in CGS group and significantly lower in Dys and ICM group than that in GC group(45% and 38%,32% and 32%,60% and 70%,P<005);meanwhile these two rates were significantly higher in Hp positive patients than that in Hp negative ones of GC,Dys and ICM groups(P<005 or P<001).After Hp eradicative treatment,the number of eradicated patients whose positive expression of both ras p21 and mutant p53 became negative increased significantly than that of not yet eradicated ones(P<005 or P<001).Conclusion:Hp infection may play an important role in the activation of ras oncogene and mutation of p53 cancer suppressive gene,which may be one of the mechanisms of carcinogenesis.
出处
《新医学》
1998年第9期469-470,473,共3页
Journal of New Medicine
基金
广东省卫生厅医学科研课题
关键词
幽门螺杆菌
癌基因
癌前病变
胃肿瘤
Helicobacter pylori Oncogene Precancerosis Stomach neoplasm
