摘要
目的观察１％Ｌ－精氨酸（Ｌ－ａｒｇ）饮食对５／６肾大部切除大鼠（ＳＮｘ）残余肾代偿性增生（ＣＲＧ）的影响。方法实验分组：（１）假手术（ｓｈａｍ）组；（２）ｓｈａｍ＋Ｌ－ａｒｇ组；（３）ＳＮｘ组；（４）ＳＮｘ＋Ｌ－ａｒｇ组。大鼠于术后３０天处死，观察Ｌ－ａｒｇ对残余肾湿重（ＫＷ）、肾重／体重比（ＫＷ／ＢＷ）、ＣＲＧ、残余肾蛋白质、ＤＮＡ含量、细胞增生以及平均肾小球体积（ＶＧ）的影响。结果Ｌ－ａｒｇ明显增加ＫＷ（Ｐ＜０．０５）、ＫＷ／ＢＷ（Ｐ＜０．０５）、ＣＲＧ（Ｐ＜０．０５）以及残余肾蛋白质、ＤＮＡ含量（Ｐ分别＜０．０１，０．００１）。免疫组化显示，残余肾小管、间质增殖细胞核抗原（ＰＣＮＡ）阳性细胞数明显增加（Ｐ＜０．０５）。此外，ＳＮｘ＋Ｌ－ａｒｇ组ＶＧ较ＳＮｘ组增加１６％。结论Ｌ－精氨酸可能刺激残余肾代偿性增生。
Objective To observe the influence of L-arginine(L-arg)on the compensatory renal growth (CRG)in rats with 5/6 subtotal nephrectomy(SNx).Methods This experiment included four groups(n=5 per group):1.Sham,2 Sham+L-arg,3.SNx,4.SNx+L-arg.Rats were sacrificed at the end of one month experiment.The following parameters were studied:wet remnant kidney weight (KW),ratio of kidney weight/body weight(KW/BW),CRG,protein and DNA content of remnant kideny.The immunohistochemical staining for proliferative cell nuclear antigen(PCNA)and mean glomerular volume were also determined(VG).Results L-arg significantly increased KW (P<0.05),KW/BW(P<0.05),CRG(P<0.05)and the content of protein and DNA(P<0.01,0001 respectively)in rats with SNx compared to those without Larg treatment.Furthermore,immunostaining for PCNA showed that Larg treatment significantly increased the number of PCNA positive cells in the tubulointerstitium of remnant kidney(P<0.05).The VG was also increased 16% in L-arginine treated SNx rats.Conclusion L-arginine might potentiate the CRG of remnant kidney,which implied the potential therapeutic role of limiting L-arg supplementation on the progression of chronic renal scarring.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第4期243-246,共4页
Chinese Journal of Nephrology
