摘要
目的:用基因芯片技术分析、筛选Midkine高表达前后胃腺癌相关基因谱的差异表达。方法:建立Midkine高表达BGC823胃腺癌细胞系,提取细胞总RNA,逆转录Cy3、Cy5标记cDNA探针,应用晶芯表达谱芯片,分析差异表达的基因。结果:发现Midkine高表达前后BGC823胃腺癌细胞有显著性表达差异的基因共有550个,其中上调基因407个,下调基因143个。对550条差异表达基因进行初步功能分类分析,结果表明这些基因与Midkine促进胃腺癌发病机制存在相关性。结论:Midkine高表达前后BGC823胃腺癌细胞部分功能基因存在显著的表达差异,多数差异表达的基因参与细胞黏附、细胞骨架形成、细胞周期以及细胞代谢等过程。
Objective： To investigate and screen the difference of gene expression profile in gastric adenocarcinoma BGC823 cell before and after midkine gene over - expression by gene chip technique. Methods ： The total RNAs were isolated from BGC823 cells before and after midkine gene over - expression. The eDNA retro - transcribed from equal quantity mRNA were labeled with Cy3 and Cy5 fluorescence as probes. Analysis on differential expression genes was carried out. Results ： A total of 550 genes were screened out which expression level changed significantly after midkine over - expression. Among these genes, 407 genes were up - regulated and 103 genes were down - regulated. While these genes were classified initially according to function,it was found that they played important role in pathogenesy of gastric adenocarcinoma promoted by midkine. Conclusion： Significant differences are found in the expression of some functional genes in BGC823 cells before and after midkine over - expression. Most of these genes are involved in cell adhesion, cytoskeleton, cell cycle and metabolism.
出处
《现代肿瘤医学》
CAS
2009年第10期1825-1829,共5页
Journal of Modern Oncology
基金
国家自然科学基金项目(编号:30872941)
南京大学研究生科研创新基金项目(编号:2006CL09)
