摘要
目的:研究胃肠舒和血流干预药物对糖尿病胃轻瘫大鼠胃窦平滑肌细胞(SMC)病变的影响。方法:131只大鼠,随机分为正常组(10)、模型组(22)、止血敏组(33)、蚓激酶组(33)和胃肠舒组(33),除正常组外大鼠1次性ip链脲佐菌素(60 mg.kg-1)造模,以7天后血糖≥16.7 mmol.L-1为纳入标准。3天后开始分别ig胃肠舒、止血敏i、p蚓激酶,模型组和正常组予蒸馏水ig。30周后检测血糖、碘[125I]-胰岛素(FINS)、碘[125I]-C肽(C-P)、胃液体排空率等。取胃窦组织,行HE染色、Mallory染色和免疫组化染色,光镜下观察胃窦组织SMC形态变化、SMC凋亡以及凋亡调控基因Bcl-2和Fax基因蛋白的表达。电镜下观察胃窦组织SMC超微结构改变。结果:30周后模型组大鼠胃窦SMC变性,SMC凋亡指数升高,与正常组比较差异显著(P<0.01),SMC中Bcl-2基因蛋白表达减低、Fas基因蛋白过度表达,与正常组比较有显著性差异(P<0.01)。胃肠舒和蚓激酶治疗后胃窦平滑肌病变明显改善,而止血敏却使胃窦平滑肌病变加重。结论:糖尿病胃轻瘫存在着胃窦SMC病变,血流干预药物对其有影响,胃肠舒具有降糖、改善胃窦SMC病变、促进胃动力的作用。
Objective： To study the effect of ＂Weichangshu＂ and medical interfering on blood flow and gastro smooth muscle cells in rats of diabetic gastroparesis model. Methods： One hundred thirty-one male SD rats （SPF class） were randomly divided into the control group （ 10 rats）, model group （ 22 rats）, ethamsylate group （ 33 rats）, lumbrukinase group（33 rats） and WCS group（33 rats）. Rats were intraperitoneally injected streptozotocin（STZ） solution（60 mg·kg^-1）. After seven days, we established blood sugar more than or equal to 16.7 mmol·L^-1 as experimental standard. Rats in the WCS group were treated by traditional Chinese medicine ＂WCS＂. So rats in the ethamsylate group treated by ethamsylate injection, rats in the lumbrukinase group treated by lumbrukinase enteric-coated capsules, rats in the control group and rats in the model group were treated by distilled water one time per day. After having been observed for 30 weeks, blood sugar, FINS, C-P and gastric fluid emptying rate were determined. Subsequently, samples of sinus ventriculi were made HE staining, Mallory staining and immunohistichemistry staining. Pathological lesion of gastric smooth muscle cells（SMC） was examined microscopically. Other sample of sinus ventriculi was made up electron microscopic specimen so as to know pathological lesion of uhrastructure of SMC through transmission electron microscope. Results： After 30 weeks, in the model group, endochylema of gastro smooth muscle celts were degenerated. The apoptotic index of SMC in rats of the model group was（24.33 ± 2.34）% determined by TUNEL method, there was significantly difference compared with rats of the control group（P 〈0.01）. Protein expression of Bcl-2 in SMC was reduced in rats of the model group,but protein expression of Fas was overexpression, there was significantly difference compared with rats of the control group （ P 〈 0.01 ）. However, pathological lesion of gastro smooth muscle was obviously improved in rats of the WCS group and in rats of the lumbrukinase group. But in rats of the ethamsylate group pathological lesion of gastro smooth muscle was more serious. Conclusion：There were pathological Changes in gastric smooth muscle cells（SMC）in rats of diabetic gastroparesis model. Medicinal interfering in blood flow had the effect on gastric SMC pathology in rats of diabetic gastroparesis model. ＂WCS＂ could decrease blood sugar and improve gastric SMC pathology and accelerate gastrointestinal motility.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第9期71-77,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
珠海市科技计划项目(PC20052038)
关键词
胃肠舒
血流干预药物
大鼠
糖尿病胃轻瘫
胃窦平滑肌细胞(SMC)病变
Weichangshu
medicinal interfering in blood flow
rat
diabetic gastroparesis model
pathological changes in gastro smooth muscle cells
