摘要
目的:建立多种中成药中芍药苷的含量测定方法,为中国药典中含芍药苷的/中成药提供一致的测定方法。方法:采用控温25℃超声处理,50%甲醇作为提取溶剂;高效液相色谱法,采用Agilent SB-C18(4.6mm×250mm,5μm)色谱柱,以乙腈-水(16∶84)为流动相,流速1.0mL·min-1,检测波长230nm,柱温25℃,对5种剂型10个中成药中芍药苷的含量进行测定。结果:芍药苷浓度在2.095～335.2μg·mL-1范围内线性关系良好(r=0.9999);日内和日间RSD(n=3)均<0.5%;最低检测限为67.02ng·mL-1;10个中成药艾附暖宫丸、复方黄连素片、胃康灵胶囊、加味逍遥丸、抗感颗粒、气滞胃痛颗粒、千金止带丸、十全大补丸、舒肝和胃丸、通天口服液方法重复性的RSD(n=6)分别为1.0%,0.69%,0.76%,0.37%,0.63%,0.57%,0.65%,0.33%,0.62%,0.60%;加样回收率(n=6)结果分别为102.1%,103.4%,98.64%,100.6%,101.6%,97.32%,98.49%,100.8%,101.2%,100.1%,RSD分别为0.23%,0.21%,0.22%,0.24%,0.32%,0.55%,0.87%,0.39%,0.51%,0.13%。结论:本法快速简便,重复性好,测定结果准确可靠,能够用于中成药中芍药苷的含量测定。
Objective：To establish and unify a method for the determination of paeoniflorin in traditional Chinese medicine patent preparations. Methods：The samples were extracted by ultrasonic treatment at 25 ℃ with 50% methanol. HPLC condition：an Agilent SB -C18 column（4.6 mm× 250 mm,5 μm） was adopted with the mobile phase consisting of acetonitrile -water（ 16： 84） at a flow rate of 1.0 mL ·min- 1, the detecting wavelength was 230 nm, and the column temperature was 25 ℃. Ten samples of five dosage forms were determined by this method. Results：The calibration curve was linear within the range of 2. 095 - 335.2 μg· mL-1 for paeoniflorin （r = 0. 9999 ） ; RSDs of intra - day and inter - day precisions at low, middle and high concentration were all less than 0.5 % ; The limits of detection was 67.02 ng· mL - 1. The reproducibility （ RSD, n = 6 ） of Aifu Nuangong pills, compound Huangliansu pills, Weikangling capsules, Jiawei Xiaoyao pills, Kanggan granules, Qizhi Weitong gran- ules,Qianjin Zhidai pills, Shiquan Dabn pills, Shugan Hewei pills, Tongtian oral liquid are 1.0%, 0. 69%, 0.76% ,0. 37% ,0.63% ,0.57% ,0.65% ,0. 33% ,0.62% ,0.60% respectively; The recoveries（ n = 6 ） were 102.1% ,103.4% ,98.64% ,100.6% ,101.6% ,97.32% ,98.49% ,100.8% ,101.2% ,100. 1% ,RSDs were 0. 23% ,0. 21% ,0. 22% ,0. 24% ,0. 32% ,0.55% ,0. 87% ,0. 39% ,0.51% ,0. 13%. Conclusion：The meth- od is rapid, accurate, sensitive, reproducible, and can be used for the determination of paeoniflorin in traditional Chinese medicine patent preparations.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第9期1440-1443,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱法
芍药苷
中成药
含量测定
HPLC
paeoniflorin
traditional Chinese medicine patent preparations
determination
