摘要
目的:研究扶正解毒汤(FJD)对硫酸镍(NiSO4)诱导大鼠肾细胞系(NRK)微核形成的抑制作用。方法:以NiSO4、FJD含药血清处理NRK细胞,检测微核率的变化及细胞总的清除自由基抗氧化力(TRAC)。结果:NiSO4(400μmol/L)暴露组细胞微核率、TRAC明显下降,低、中、高剂量FJD血清与NiSO4共处理组细胞微核率显著低于NiSO4暴露组(P<0.05,P<0.01),细胞TRAC则显著提升(P<0.05,P<0.01)。结论:NiSO4可诱发细胞微核形成,FJD对镍诱导NRK细胞微核形成有明显抑制作用,其机制可能与抗氧化应激有关。
Objectives： To study the inhibitory effect of Fuzheng Jiedu Decoction（FJD） on the micronuclei formation induced by nickel sulfate（NiSO4） in rat renal cell line（NRK）.Methods：Cultured NRK cells were exposed to NiSO4（400μmol/L） and FJD drug serum.Micronuclei（MN） were scored in 1000 NRK cells and cellar total radical-trapping antioxidative capacity（TRAC）was measured.Results： Compared with NiSO4 group,the MN frequencies decreased and TRAC increased in groups treated with both FJD and NiSO4 significantly （ P 〈 0.05, P 〈 0.01 ）. Conclusion ： FJD could suppress nickel-induced micronuclei formation in NRK ceils which was probably related to its antioxidant property.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2009年第8期591-592,595,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
教育部博士点专项科研基金(20050730031)
关键词
扶正解毒汤
硫酸镍
微核
肾细胞
总抗氧化力
Fuzheng Jiedu decoction
nickel sulfate
micronuclei
renal cell
total radical-trapping antioxidative capacity（TRAC）
