摘要
目的建立高效液相色谱法测定延胡索酸泰妙菌素含量。方法采用Agilent C18(150mm×4.6mm,5μm),流动相为0.1mol·mL-1碳酸铵缓冲液(取碳酸铵10g,加水800mL使溶解,加24mL6%的高氯酸水溶液,加水至1000mL)-乙腈-甲醇(27∶23∶50);检测波长为212nm。结果线性范围为0.25～0.75mg·mL-1(r=0.9994);平均回收率为99.3%(RSD=1.2%)。结论方法快捷,准确,重复性好。
OBJECTIVE To establish a method for determination the content of tiamulin fumarate. METHODS RP-HPLC was adopted, the column was Agilent C18 （150 mm×4.6 mm, 5 μm）, the mobile phase was 0.1 mol·mL^-1 ammonium carbonate buffer （add 10 g ammonium carbonate to a 1 000 mL volumetric flask and dissolve in about 800 mL of water. Add 24 mL 6% of perchloric acid and dilute with water to volume） -acetonitril-methanol （27 ： 23 ： 50）. UV dector wavelength was 212 nm. RESULTS The calibration is linear between 0.25-0.75 mg·mL^-1. The mean recovery was 99.3%, RSD%=1.2%. CONCLUSION This method inspecting tiamulin fumarate is rapid, accurate and reproducible.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第9期766-768,共3页
Chinese Journal of Modern Applied Pharmacy
