摘要
利用格网和重点混合抽样技术结合GPS采集了成都市郊区27个样点的表层土样,用常规方法对其芽孢杆菌进行分离、计数和鉴定,在ArcGIS9.0平台上运用地统计学方法研究了该地区土壤芽孢杆菌的空间分布特征。结果表明,成都郊区土壤芽孢杆菌较丰富,数量在4.30×103～8.53×105cfu.g-1干土。空间分布总体上呈条带状分布和局部团块状,从西南向东北方向呈递增趋势。以大于平均数为评估值的概率分布图呈条带状和局部团块状,从南向北,从西向东概率逐渐递增。从27个土样中共分离到21个种,环状芽孢杆菌、巨大芽孢杆菌、地衣芽孢杆菌、蜡状芽孢杆菌为区域优势种群。成都郊区芽孢杆菌的物种丰富度(S)、物种多样性指数(H′)和均匀度指数(R)均较高,分别为7.259±1.375、1.368±0.237和0.698±0.115,而生态优势度指数(D)较低(0.319±0.094)。综上表明,成都郊区土壤芽孢杆菌比较丰富,种群间相对平衡,以一种或少数几种芽孢杆菌控制区域环境的情况不明显。
By using the approaches of grid- and stratum sampling, twenty seven 0-20 cm soil samples located by GPS were collected from Chengdu suburbs to study the spatial distribution and species diversity of Bacillus spp. The isolation, counting, and identification of Bacillus spp. were made with conventional methods, and the spatial variability was performed by the software Arc- GIS 9. 0 with geostatistical component. The total number of culturable Bacillus spp. was comparatively high, being 4. 30 × 10^3-8.53 × 10^5 cfu · g^-1 dry soil, and the Bacillus mainly exhibited strip and partially aggregative spatial distribution patterns. The individuals of soil Bacillus in- creased gradually from southwest to northeast, and the spatial probability distribution of Bacillus spp. （ 〉 1. 693 × 10^5 cfu · g^-1 dry soil） mainly presented strip or partially aggregative shape, with the probability grade increased gradually from south to north and from west to east. Based on the characteristics of morphology and physiology/biochemistry, 21 Bacillus species from 333 isolates were identified, among which, B. circulans, B. megaterium, B. licheniformis, and B. cereus were the dominant species. The richness index, species diversity index and evenness index were high, being 7. 259 ± 1. 375, 1. 368 ± 0. 237 and 0. 698 ± 0. 115, respectively, but the ecological dominance index was low （0. 319 ± 0. 094）, which suggested that in Chengdu suburbs, soil Bacillus spp. were comparatively abundant and had a relative balance among the species, but the prevalent species were not obvious.
出处
《生态学杂志》
CAS
CSCD
北大核心
2009年第10期2009-2013,共5页
Chinese Journal of Ecology
基金
四川省教育厅项目(01LA02和2004A2002)
四川农业大学校科技基金资助项目(1301)
