摘要
目的:建立以反相高效液相色谱法测定喘敷灵巴布剂中芥子碱硫氰酸盐含量的方法。方法:采用Aichrom Bond-AQC_(18)(250 mm×4.6mm,5μm)色谱柱;流动相:乙腈-0.1 mol·L^(-1)磷酸二氢钾(18:82);检测波长为326nm;流速:1.0ml·min^(-1);柱温:25℃。结果:芥子碱硫氰酸盐在0.4～2.0μg之间具有良好的线性关系,平均加样回收率为98.55%,RSD=1.29%。结论:该方法简便可靠,具有很好的重复性和稳定性,可用于喘敷灵巴布剂中芥子碱硫氰酸盐含量的质量控制。
Objective： To establish a method for determaination of sinapine cyanide sulfonate in chuanfuling cataplasm by RP- HPLC. Method： Aichrom Bond-AQ C18 column （250 mm × 4.6mm, 5μm）was used; Mobile phase consisted of acetonitrile-0. 1 mol·L^-1 potassium dihydrogen phosphate（ 18： 82） ; Detection Wavelength was 326nm; Flow rate was 1.0 ml·min^-1; column temperature was 25℃. Result： Sinapine cyanide sulfonate had good linear relation between 0. 4 - 2. 0ug, the average sample recovery was 98.55%, RSD = 1.29%. Conclusion： The method is not only accurate and rapid, but also reproducible and steady ,which fits to control the amount of sinapine thiocyanate in chuanfuling cataplasm.
出处
《中国药师》
CAS
2009年第10期1407-1408,共2页
China Pharmacist
