摘要
目前原料乳中蜡样芽孢杆菌的检测方法繁琐费时耗力,针对此缺点建立一种操作简便快速检测方法,采用过滤富集菌体后PCR技术来检测原料乳中蜡样芽孢杆菌。整个过程只需6 h左右即可完成,检测灵敏度高达101 mL-1。这种方法是对传统检测方法的有效改进,并且达到了原料乳检测快速、准确、灵敏的要求。
Conventional methods of detecting Bacillus cereus in raw milk are cumbersome and time-consuming. To establish a time-saving detecting method, pathogen was concentrated from raw milk by straightforward filtration and was detected by a rapid and sensitive PCR system for detection of Bacillus cereus. The assay from filtration to PCR can be completed in about 6 h and the limit of detection for PCR was as low as 10 cfu of bacteria in 1 mL of raw-milk. The study, which increases the accuracy and sensitivity, improves traditional methods and it can be applied to the food microorganism detection and diagnosis of food poisoning.
出处
《中国乳品工业》
CAS
北大核心
2009年第9期43-45,共3页
China Dairy Industry
基金
国家科技支撑计划重点项目(2009BADB9B06)
黑龙江省普通高等学校青年学术骨干支持计划项目(1151G006)
