摘要
脐血中含有丰富的造血干细胞和间充质干细胞,脐血采集有体内采集法和体外采集法两种方法。脐血中所有的干细胞尽可能的回收是脐血处理过程中关键的一步。脐血中单核细胞的分离也是建立脐血库和脐血干细胞移植的关键环节。脐血分离方法有密度梯度离心法、羟乙基淀粉沉降法、单克隆抗体流式细胞仪分析法、免疫磁珠分选法等,不同方法各有优劣。密度梯度离心结合直接贴壁法可在体外分离培养人脐血中的间充质干细胞。利用生物反应器可大规模扩增人脐血造血干/祖细胞。人脐血中的间充质干细胞,在一定的条件下能向肝细胞定向分化。脐血的冻存主要采用程控降温法。玻璃化法超低温保存以其简单、快速、高效等优点,在脐血干细胞的长期保存方面展现了良好应用前景。脐血造血干细胞移植具有诸多优点,广泛应用于临床各种疾病的治疗中。
Umbilical cord blood that is rich in hemopoietic stem cells and mesenehymal stem cells is collected in vivo and in vitro. Reclamation of stem cells plays an important role in processing umbilical cord blood; additionally, separation of monocytes also plays an important role in establishing bank of umbilical cord blood and performing umbilical cord blood stem cell transplantation. Umbilical cord blood is separated using density gradient centrifugation, hydroxyethyl starch settling process, monoclone antibody flow cytemetry, and immunomagnetic bead sorting. Density gradient centrifugatien combined with adherent method is used to in vitro separate and culture mesenehymal stem cells; bio-reactor is used to amplify human umbilical cord blood stem cells and progenitor cells. Under a certain condition, mesenehymal stem cells are able to differentiate into hepatocytes. Cryopreservation is mainly used to freeze umbilical cord blood. Vitrification method characterizes by sample, rapid, and high effective, thus it is an ideal method to maintain umbilical cord blood stem cells for a long term. Umbilical cord blood stem cell transplantation benefits for treating various diseases in clinic.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第36期7197-7200,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
