摘要
目的观察甲型流感病毒FM1感染小鼠体内流感病毒复制量的不同,研究中药复方银翘柴桂汤体内抗甲型流感病毒的作用。方法NIH小鼠分为正常对照组、病毒模型组、利巴韦林组及银翘柴桂汤组,除空白对照组外,将甲型流感病毒FM1稀释液滴鼻感染各组小鼠,建立流感病毒感染模型。病毒感染前1 d及感染后4 d,银翘柴桂汤组及利巴韦林组分别灌胃银翘柴桂汤和利巴韦林,病毒感染后第5天处死小鼠,解剖,提取肺组织总RNA,实时荧光定量PCR(RT-PCR)方法测定小鼠肺组织流感病毒复制量。结果正常对照组无扩增曲线,Ct>30,流感病毒病原体检测阴性;银翘柴桂汤组、利巴韦林组及病毒模型组扩增曲线Ct分别为19.49±1.40、21.02±4.25、15.80±4.18,流感病毒病原体检测阳性。与病毒模型组病毒复制量(128 973.40±18.14)相比,利巴韦林组病毒复制量(33 327.29±19.56)降低,有显著差异性(P<0.05);银翘柴桂汤组病毒复制量(79 104.28±2.52)降低,有显著性差异(P<0.05)。结论建立的流感病毒感染模型可靠,银翘柴桂汤对流感病毒感染小鼠肺内流感病毒增殖具有明显抑制作用,有抗流感病毒作用。
Objective To observe the quantity of influenza virus replication in mice inflected influenza A virus FM1, and study the effect of Yinqiaochaigui Decoction on influenza A virus in vivo. Method NIH mice were randomly divided into the normal control group, virus model group, ribavirin group and Yinqiaochaigui Decoction group. The influenza virus inflection model was established by nasal drip of influenza A virus FM1 dilution in all groups except the normal control group. The Yinqiaoehaigui Decoction group and ribavirin group were given intragastrically Yinqiaochaigui Decoction and ribavirin one day before and 4 days after the virus infection respectively. After one day the mice were killed and dissected and the total RNA of lung tissue was extracted. The quantity of influenza virus replication in mouse lung tissue was detected by using real-time fluorescence quantitative polymerase chain reaction （RT-PCR）. Result The normal control group had no amplification curve （Ct 〉 30） and the result of virus pathogen detection was negative. In the Yinqiaochaigui Decoction group, ribavirin group and virus model group Ct was 19.79 ±1.40, 21.02 ± 4.25 and 15.80 ± 4.18 respectively. The result of virus pathogen detection was positive. The quantity of influenza virus replication decreased in the ribavirin group （33 327.29 ± 19.56） compared with that in virus model group （128 973.40 ± 18.14） and the difference was significant （P 〈 0.05）. The quantity of influenza virus replication in Yinqiaochaigui Decoction group decreased （79 104.28 ±2.52） compared with that in the virus model group and the difference was significant （P 〈0.05）. Conclusion The model is reliable. Yinqiaochaigui Decoction has significant inhibitory effect on the proliferation of influenza virus and it has anti-virus effect in infected mice.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第8期540-542,I0001,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家科技部"十一五"科技支撑计划资助项目(No.2006BA101B00)
关键词
银翘柴桂汤
流感病毒
实时荧光定量PCR
小鼠
Yinqiaochaigui Decoction
influenza virus
real-time fluorescence quantitative polymerase chain reaction
mice
