摘要
以子囊菌油脂酵母(Lipomyces tetrasporus)NRRL Y11562为出发菌株,经紫外诱变选育出1株高产油脂的优良酵母UV5菌株.对该菌种的发酵培养基和发酵条件进行优化,发现当以葡萄糖为碳源,硫酸铵为氮源,C/N为308,接种量为10%,初始pH为6.5,培养温度为28℃,培养96 h时,油脂产率最高,达9.09 g?L-1.用气相色谱法对发酵得到的油脂进行脂肪酸组成分析,结果显示该突变株产生的油脂主要由软脂酸(C16:0)、棕榈油酸(C16:1)、硬脂酸(C18:0)、油酸(C18:1)和亚油酸(C18:2n-1)组成,其脂肪酸组成与植物油近似.
The UV-induced mutant strain UV5, obtained by UV-induced mutagenesis of Lipornyces tetrasporus NRRL Y11562, showed the excellent capability of accumulating lipid. Several factors influencing lipid production of UV5 were studied. following conditions： shaking flask fermentation, The lipid productivity reached 9.09 g. L-1 under the glucose as carbon source, （NH4）2SO4 as nitrogen source, ratio of carbon to nitrogen at 308, initial pH 6. 28 ℃ for 96 h. Fatty acid compositions of the produ showing the primary compositions of palmitic 0）, oleic acid（C18：1）and linoleic acid（C18：2n oil. inoculation proportion of 10%, incubated at oil were analyzed by gas chromatography, 0）, palmitoleic acid（Cl6：1）, steric acid（C18 ：1）, and these compositions were similar to the vegetable
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第5期497-502,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
关键词
油脂酵母
发酵条件
油脂组成及含量
oleaginous yeast
fermentation condition
lipid composition and content
