摘要
研究LachnumYM-223黑色素的发酵工艺及其抗氧化活性。在单因素试验的基础上进行正交试验,得出最佳发酵条件为:土豆汁200g/L、葡萄糖20g/L、蛋白胨5.0g/L、酪氨酸1.0g/L、硫酸铜0.32g/L、pH7.0、200r/min、25℃恒温振荡培养12d。在此发酵条件下黑色素的产量提高了53.6%。黑色素浓度为0.5g/L时还原力接近0.05g/LVC体系;在1.0g/L时,对·OH的清除率高达50.6%;黑色素添加量为大豆油的0.05%时,可明显抑制大豆油的自氧化作用。作为一种新型的天然色素,YM-223黑色素有望应用于食品、化妆品以及医药等行业。
The fermentation conditions of Lachmum YM-223 including medium composition and period were optimized to achieve the maximum melanin production using single-factor and orthogonal array methods and the melanin obtained was subjected to analysis of antioxidant activities such as reducing power, · OH radical scavenging activity and inhibition activity towards autoxidation of soybean oil. When Lachraum YM-223 was cultured using a medium consisting of potato juice 200 g/L, glucose 20 g/L, tryptone 5.0 g/L, tyrosine 1.0 g/L and CuSCh 0.32 g/L at initial pH 7.0 with shaking speed 200 r/min at 25℃ for 12 d, the melanin yield was up to the maximum, higherthan that using basic culture medium by 53.6%. The reducing power of 0.5 g/L melanin was equivalent to 0.05 g/L VC system and the scavenging effect of 1.0 g/L melanin on ·OH radicals reached 50.6% and the autoxidation of soybean oil was obviously inhibited by the addition of 0.05% melanin. The melanin produced by Lachmum YM-223 can be used as a widespread new-style natural pigment in food, cosmetics and medicine industry.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第17期185-189,共5页
Food Science
基金
安徽省自然科学基金项目(090413086)
关键词
粒毛盘菌
黑色素
发酵
抗氧化活性
Lachmum
melanin
fermentation
antioxidant activity
