摘要
通过选用与血脂代谢及肥胖、肿瘤细胞增殖相关的PPARs模型对羊肚菌多糖PMEP-1的生物活性进行高通量药物筛选(high-throughput dcreening,HTS),结果发现:PMEP-1对PPARδ模型有一定的激活作用,但作用很弱,效果不显著;对PPARα模型没有激活作用。对羊肚菌多糖PMEP-1降血脂作用进行动物实验,结果发现:各剂量组(低剂量100mg/kg·d、中剂量200mg/kg·d、高剂量400mg/kg·d)大鼠血清甘油三酯(TG)显著地低于高脂模型组(P<0.05),低、中剂量组大鼠血清低密度脂蛋白胆固醇(LDL-C)和动脉粥样硬化指数(AI)显著地低于高脂模型组(P<0.05),而对大鼠血清总胆固醇(TC)和高密度脂蛋白胆固醇(HDL-C)没有显著影响,说明PMEP-1具有降血脂功能,对高脂血症的发生有一定的预防作用,同时对冠心病及动脉粥样硬化也有一定的预防作用。
The possible biological activities of a water soluble polysaccharide fromMorchella esculenta （L.） Pets. named PMEP-1 were tested with peroxisome proliferator-activated receptor （PPAR） models related to blood lipid metabolism, and proliferation of tumor cells by high-throughput screening （I-ITS）. PMEP-1 had sfight activation effects on PPAR δ models but no effects on PPAR α models. Animal experiments showed that TG in experiment groups 0ow dose 100 mg/kg · d, medium dose 200 mg/kg · d and high dose 400 mg/kg·d） was significantly （P〈 0.05） lower than that in hyperlipidemic model group, the LDL-C content and the Al in low- and medium-dose groups were significantly （P〈 0.05） lower than those in hyperlipidemic model group, while no significant differences in TC and HDL-C were seen among all the experiment groups and hyperlipidemie model group. So we concluded that PMEP-1 has hyperlipidemic acticity, and can prevent hyperlipidemia, coronary disease and atherosclerosis
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第17期285-288,共4页
Food Science
基金
西南大学博士基金项目(SWU109005)
云南省省校省院科技合作支持项目(2005YX24)
关键词
羊肚菌
多糖
降血脂
高通量筛选
Morchella esculenta （L.） Pers
polysaccharide
hyperlipidemic activity
HTS
