摘要
目的:探讨中药连翘对严重烧伤大鼠的免疫调节作用及其机制。方法:采用大鼠30%体表面积Ⅲ度烧伤模型。实验共分5组,分别为正常对照组(Control),烧伤后8h组(8PBH),连翘高、中、低剂量组(AFS1、AFS2、AFS3),其中AFS1、AFS2和AFS3组在烧伤前7d开始灌胃给药,1次/d,分别给予AFS5g/kg、2.5g/kg和1.25g/kg。各组在烧伤后8h留取外周血和脾脏组织,流式细胞术测外周血Treg百分数;RT-PCR和免疫组化法测定脾脏组织Foxp3表达。结果:与Control组比较,8PBH组外周血Treg水平显著升高,脾脏组织Foxp3mRNA和蛋白表达明显上调;AFS1、AFS2及AFS3组均能显著减轻上述指标变化,并呈剂量依赖关系。结论:连翘具有免疫调节作用,其作用机制与干扰Foxp3基因的表达有关。
AIM： To explore the immunity modulation function of aqueous of Forsythia suspense （AFS） and its possible mechanisms. METHODS： Rats of burned model group were burned with vapor under 3mpa pressure and 108℃ temperature for 8 seconds to achieve deep partialthickness burn, to make a thirty percent total body surface area（TBSA） burn. The experiment were divided into five groups： Control group： without any treatment; 8 PBH group： 8 h after burn; the rats of AFS1 guoup, AFS2 group and AFS3 group of them were given AFS 5 g/kg, 2.5 g/kg, 1.25 g/kg once a day by Po. pathway for seven days before burns, respectively. Rats were sacrificed before and 8h after burn, The percentage of Treg cells in CD4^ ＋ T cells was detected by flow cytometry（FCM） ; the expression of Foxp3 mRNA on splenocytes were measured by RT-PCR, and the protein of Foxp3 activity was evaluated by immunohistochemistry staining. RESULTS： Compared with Control group, the expression of Foxp3mRNA and protein on the splenocytes were upregulated markedly（ P 〈0. 01 ）, and the percentage of Treg were significantly increased （ P 〈 0. 0l ） in the 8PBH group. AFS1, AFS2 and AFS3 significantly attenuated these increases （ P 〈 0.01 ）, which was dose-dependent. CONCLUSION： AFS has immunity modulation function and mechanism of it is corrected with Foxp3 mRNA on splenocytes.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第10期935-937,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
江西省自然科学基金资助项目(2007GZY1009)
