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In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f. 被引量:6

In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f.
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摘要 In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg.L^-1) and NAA (0.1 mg.L^-1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg.L^-1) and NAA (0.1 mg.L^-1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg.L^-1) and activated charcoal (0.5 mg.L^-1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii. In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg.L^-1) and NAA (0.1 mg.L^-1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg.L^-1) and NAA (0.1 mg.L^-1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg.L^-1) and activated charcoal (0.5 mg.L^-1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii.
作者 CHEN Bi-hua
出处 《Forestry Studies in China》 CAS 2009年第3期174-178,共5页 中国林学(英文版)
基金 supported by the Youth Talent Project of Science and Technology Department, Fujian Province (No. 2007F3017) the Research Project of the Forestry Department, Fujian Province (Minlin 2004 Kehan No. 8)
关键词 in vitro propagation Tripterygium wilfordii Hook f. MULTIPLICATION ROOTING activated charcoal in vitro propagation, Tripterygium wilfordii Hook f., multiplication, rooting, activated charcoal
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