摘要
[目的]为转ChIFN-γ烟草通过口服喂养途径进行免疫应用提供试验依据。[方法]将转ChIFN-γ基因烟草叶片添加到饲料中饲养雏鸡,接种新城疫病毒(NDV)后观察ChIFN-γ疫苗佐剂通过口服喂养方式对雏鸡的增重效果和免疫保护作用。[结果]口服转ChIFN-γ烟草对雏鸡的体重增长有一定促进作用;喂养含转ChIFN-γ烟草叶片饲料1～10d的雏鸡对NDV均表现出不同程度的抗性,其中喂食3d3600UChIFN-γ的效果最好。[结论]该研究表明通过口服转ChIFN-γ烟草叶片来防治鸡新城疫病是可行的。
[ Objective ] The research aimed to provide experimental references for the immunity application of transgenie chicken interferon gene （ChIFN-γ） tobacco for oral administration. [ Method] Feeding chicks with feed added transgenic ChIFN-γ tobacco leaves and then observing chicks weight gain rate and the immune protective effects using ChIFN-γ plant - based vaccine adjuvant through oral feeding patterns after inoculation of Newcastle disease virus（NDV）. [ Result] The results showed that feeding transgenic ChIFN-γtobacco leaves through oral feeding patterns could increase the weight of chicks. And chicks feeding with transgenic ChIFN-γ tobacco leaves for 1 - 10 d showed different resistibilities. Among all treatments, feeding with 3600 U ChIFN-γ for 3 d was best. [ Conclusion ] This study had shown that it was feasible to prevent Newcastle disease with transgenic ChIFN-γ tobacco leaves through oral feeding patterns.
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14709-14710,14748,共3页
Journal of Anhui Agricultural Sciences
基金
国家科技支撑计划项目(2007BAD59B06)
中国国际合作资助项目(2007DFA31260)
国家大学生创新实验计划项目[贵大国创字(2008)004号]
