摘要
目的:建立制备黄芩素的新方法,并用紫外分光光度法对产品进行含量测定.方法:建立了水提法制备黄芩苷,酶解法制备黄芩素的新方法,并考察了温度、pH值、酶活单位和时间对结果的影响,优化了工艺条件.检测波长为244 nm,甲醇溶解黄芩素,利用紫外分光光度法测定其含量.结果:制得黄芩素产率达到84.2%,纯度达到93.2%,黄芩素甲醇溶液在1～24 mg/L之间与吸光度线性关系良好,回归方程为A=0.0032C(mg/L)+0.0045,相关系数R2=0.985.结论:所确定黄芩素的制备方法以及其分光光度检测法操作简便、准确,无污染,适用于黄芩素的制备及其定量测定.
AIM： To establish a new method to prepare baicalein and to determine the content of baicalein by UV-spectrophotometry. METHODS： Water was used as the extracting solvent of baicalin and enzyme was used to convert baicalin to baicalein. The effects of temperature, pH value, enzyme activity and reaction time were studied respectively and the process conditions were optimized. The detection wavelength was 244 nm and methanol was used as the solvent. The content of baicalein was determined by UV-spectrophotometry. RESULTS： The content of baicalein reached 93.23%. The linear range of determination was 1 - 24 mg/L. Regression equation： A = 0. 0032C （mg/L） ＋ 0. 0045, R^2 = 0. 985. CONCLUSION： The newly established method is convenient, practical and non-polluting. This method is suitable for the preparation and quantitative analysis of baicalein.
出处
《第四军医大学学报》
北大核心
2009年第18期1825-1827,共3页
Journal of the Fourth Military Medical University
