摘要
目的了解新疆地区鲍氏不动杆菌的氨基糖苷类修饰酶与16S rRNA甲基化基因。方法分离20株鲍氏不动杆菌,并对其进行抗菌药物敏感性试验,用PCR方法检测鲍氏不动杆菌的氨基糖苷类修饰酶与16S rRNA甲基化基因。结果13株鲍氏不动杆菌检出氨基糖苷类修饰酶基因,其检出率为65%,其中aac(3)-Ⅰ基因阳性4株,阳性率为20%、aac(3)-Ⅱ基因阳性8株,阳性率为40%、aac(6′)-Ⅰad基因阳性4株,阳性率为20%、aac(6′)-Ⅰb基因阳性0株、aac(6′)-Ⅱ基因阳性0株、ant(3″)-Ⅰ基因阳性4株,阳性率为20%、ant(2″)-Ⅰ基因阳性1株,阳性率为5%,未检出16SrRNA甲基化基因。结论新疆地区鲍氏不动杆菌存在氨基糖苷类修饰酶基因,未检出16S rRNA甲基化基因。
OBJECTIVE To understand aminoglycoside-modifying enzyme and 16S rRNA methylase gene expressions in Acinetobacter baurnannii in Xinjiang region. METHODS 20 A. baumannii strains were isolated and test the anti-bacterial drug sensitivity. PCR methods were used to test aminoglycoside-modifying enzyme and 16S rRNA methylase gene. RESULTS From thirteen A. baumannii strains detected the aminoglycoside-modifying enzyme genes, the detection rate was 65 % in which aac （3） - Ⅰ gene was positive in 4 strains （20 %）, aac （3）-Ⅱ gene in 8 strains （40%）, aac （6′）- Ⅰad gene in 4 strain （20%）, ant （3″）-Ⅰ gene in 4 strain （20%）, and ant （2″）-Ⅰ gene was positive in 1 strain （5%）. The aac （6′）-Ⅰ b and aac （6′）-Ⅱ gens and were not detected; the 168 rRNA gene methylation was negative. CONCLUSIONS There are aminoglyeoside-modifying enzyme genes existing in A. baumannii, no 16S rRNA methylase gene was detected in Xinjiang region.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第20期2675-2677,共3页
Chinese Journal of Nosocomiology
