摘要
目的评价两种不同的三硝基丙酸(3-Nitropropionic Acid,3-NP)给药方式所致的亨廷顿氏病(Huntington’sdisease,HD)亚急性动物模型的作用。方法通过对24只SD大鼠连续给药和间断给药方式进行比较,连续给药组3-NP 15 mg/kg/d连续5 d腹腔注射;间断给药组给予3-NP 15 mg/kg/d腹腔连续注射2 d后,间隔2 d,再连续注射3 d。结果改良的间断给药方式可明显降低动物死亡率(25%);并且通过Fluro-Jade B染色,可迅速特异地检测到纹状体变性的神经元。结论三硝基丙酸间断给药方式可有效建立HD动物模型。
Objective To evaluate the efficiency of two different administrations of 3-Nitropropionic acid （3-NPA） for inducing rat model of Huntington＇s disease. Methods Twelve SD rats were divided into two groups, one receiving continuous administration of 3-NPA （15 mg/kg daily, ip, for 5 days） and the other receiving intermittent administration （ 15 mg/kg daily, ip, for 2 days, no treatment for 2 days, and then 15 mg/kg daily, ip, for 3 days）. Results Mortality rate was decreased to 25 % and the degenerating cell bodies and affected fibers in striatum were found rapidly by Fluro- Jade B staining in the intermittent administration group. Conclusion The intermittent administration of 3-NPA is an effective method for inducing animal model of Huntington＇s disease.
出处
《实验动物科学》
2009年第4期12-15,共4页
Laboratory Animal Science
关键词
三硝基丙酸
亨廷顿氏病
动物模型
3-Nitropropionic acid
Huntington＇ s disease
Animal model
