摘要
目的探讨羟基磷灰石膜复合材料上人血管内皮细胞分泌功能的变化。方法将人血管内皮细胞与纳米相羟基磷灰石（HA）薄膜复合材料体外复合培养，进行形态学和功能测定，同时分别测定HA材料组和空白对照组中血管内皮细胞分泌一氧化氮（NO）、前列环素（PGI2）水平。结果人血管内皮细胞能在纳米相羟基磷灰石薄膜复合材料上良好地黏附、增殖、生长。人脐静脉血管内皮细胞分泌NO和PGI2在HA材料组和空白对照组差异无统计学意义（P〉0．05）。结论相羟基磷灰石膜复合材料上人血管内皮细胞分泌功能正常，说明纳米相羟基磷灰石薄膜复合材料具有良好的细胞相容性，可作为组织工程化机械瓣膜材料。
Objective To study the secretion function change of human vascular endothelial cells in nanometerie hydroxyapatite （HA） film complex materials. Methods Human vascular endothelial cells and nanometefic HA film complex materials were co-cultivated in vitro. Morphological and functional indexes of human vascular endothelial cells were measured. The levels of nitric oxide （NO） and PGI2 secreted by vascular endothelial ceils of HA group and normal control group were also measured. Results Human vascular endothelial cells eould well adhere to nanometeric HA film complex materials and could well multiply and grow. The level of NO and PGI2 secreted by human vascular endothelial cells had no significant difference （P〉0.05）. Conclusion The secretion function of human vascular endothelial cells in nanometerie hydroxyapatite （HA）film are Normal. Material has good biocompatibility and could be used as tissue engineering artificial mechanical valve material.
出处
《中国心血管病研究》
CAS
2009年第6期450-453,共4页
Chinese Journal of Cardiovascular Research
基金
安徽省人才培养及科研带头人专项基金（2005）58号
关键词
脉冲激光沉积
羟基磷灰石薄膜
组织工程
人血管内皮细胞
细胞培养
血管内皮细胞功能
Impulse laser sediment（PLD）
Hydroxyapatite（HA） film
Tissue engineering
Human blood vascular endothelial cell
Cell eulture
Function of endothelial cells
